CD2 Molecule (CD2) antibody
| Antigen |
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| Synonyms |
T11, SRBC, FLJ46032, CD2, MGC140284, CD2R, LFA2, OX34, LFA-2, OX-34, Ly37, Ly-37 |
| Clonality |
Monoclonal (LT2) |
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Host
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Reactivity
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Human (146), Mouse (Murine) (54), Rat (Rattus) (46), Horse (Equine) (18), Pig (Porcine) (18), Dog (Canine) (15), Cow (Bovine) (10), Sheep (Ovine) (9), Monkey (4), Chicken (1), Goat (1)
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Application
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 Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Functional Studies (Func), Immunoprecipitation (IP)
Flow Cytometry (FACS) (192), Immunohistochemistry (Frozen Sections) (IHC (fro)) (56), Immunoprecipitation (IP) (50), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (44), Immunofluorescence (IF) (21), Western Blotting (WB) (21), Functional Studies (Func) (17), ELISA (7), ELISA (Detection) (5), Immunocytochemistry (ICC) (4), Cytotoxicity Test (CyTox) (2), Enzyme Immunoassay (EIA) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (IHC) (2), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN119499 |
| Quantity |
1 mg (1.0 mg/ml) (Variants) |
| Price |
820.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Alternative name
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CD2
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Swiss-Prot
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P06729
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Immunogen
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Normal human peripheral blood lymphocytes. Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63.653 myeloma cell line
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Isotype
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IgG2b (Matching secondary antibodies)
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Clone
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LT2
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Description
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CD2 is a single chain transmembrane glycoprotein of 50kD, expressed by T cells, NK cells and thymocytes. It acts as a receptor for CD58 (LFA-3), and is important in mediating the interaction between T cells and target cells or antigen presenting cells. Alternate Names: LFA-2, E-ROSETTE RECEPTOR.
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Specificity
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LT2 binds highly specific to human CD2. This antibody inhibits NK cell mediated cytotoxicity, and ConA and PHA mediated blast transformation.
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Application Notes
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Flow cytometry: 1/50 - 1/100, use 10 ul of the suggested working dilution to label 10e6 cells in 100ul. Immunoprecipitation. Functional Studies. Immunohistochemistry on frozen sections: 1/200 - 1/400. Recommended positive control tissue: Human Tonsil. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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1.0 mg/ml
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Buffer
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PBS, pH7.4
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Storage
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Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
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Research Area
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Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells
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Restrictions
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For Research Use only
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Publications
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Moingeon, Chang, Sayre et al.: "The structural biology of CD2." in: Immunological reviews, Vol. 111, pp. 111-44, 1990 (PubMed).
Beyers, Barclay, Law et al.: "Activation of T lymphocytes via monoclonal antibodies against rat cell surface antigens with particular reference to CD2 antigen." in: Immunological reviews, Vol. 111, pp. 59-77, 1990 (PubMed).
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Alternatives for antigen "CD2 Molecule (CD2)", type "Antibodies"
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Hosts
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Mouse (164), Rat (40), Rabbit (25)
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Reactivities
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Human (146), Mouse (Murine) (54), Rat (Rattus) (46), Horse (Equine) (18), Pig (Porcine) (18), Dog (Canine) (15), Cow (Bovine) (10), Sheep (Ovine) (9), Monkey (4), Chicken (1), Goat (1)
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Applications
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Flow Cytometry (FACS) (192), Immunohistochemistry (Frozen Sections) (IHC (fro)) (56), Immunoprecipitation (IP) (50), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (44), Immunofluorescence (IF) (21), Western Blotting (WB) (21), Functional Studies (Func) (17), ELISA (7), ELISA (Detection) (5), Immunocytochemistry (ICC) (4), Cytotoxicity Test (CyTox) (2), Enzyme Immunoassay (EIA) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (IHC) (2), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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FITC (29), Biotin (25), PE (23), RPE (8), APC (7), Alexa Fluor 488 (3), PE,Cy5 (3), Alexa Fluor 647 (2), Cy5,PE (2), DY647 (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 555 (1), Alexa Fluor 700 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Dyomics 647 (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5.5 (1), PE,Cy7 (1), Pacific Blue (1), RBITC (1), RPE,Cy5 (1)
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