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CD8 antibody (CD8a Molecule) (FITC)

Details for Product anti-CD8A Antibody No. ABIN119507, Supplier: Log in to see
Antigen
  • cd8
  • LOC100136450
  • BB154331
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • zgc:136643
  • CD8
  • Leu2
  • MAL
  • p32
  • RHACD8-4
Alternatives
anti-Human CD8 antibody for Immunohistochemistry (Frozen Sections)
Reactivity
Human
765
405
130
68
28
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25
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20
16
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12
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10
9
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8
3
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2
2
2
2
1
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1
Host
Mouse
961
392
160
Clonality (Clone)
Monoclonal ()
Conjugate
This CD8 antibody is conjugated to FITC
243
189
122
86
30
30
28
18
18
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13
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Application
Flow Cytometry (FACS)
1177
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234
189
136
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105
83
71
38
33
25
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14
10
7
7
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6
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4
4
2
2
2
1
1
1
1
1
1
1
1
1
Supplier
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Immunogen Normal Human peripheral blood lymphocytes. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63.653 myeloma cell line.
Clone LT8
Isotype IgG1
Specificity This antibody recognises the CD8 cell surface glycoprotein.
Purification Ion Exchange Chromatography.
Alternative Name CD8 (CD8A Antibody Abstract)
Background CD8 is expressed by a subset of peripheral blood T cells which express cytotoxic/suppressor activity. It is also expressed weakly on NK cells. The CD8 antigen is a co-receptor for MHC Class I in conjunction with the T cell receptor, and is important in the selection process of CD8+ MHC Class I restricted T cells.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2
Gene ID 925
NCBI Accession NP_001759
UniProt P01732
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling
Application Notes Flow cytometry: Neat - 1/5, use 10 μL of the suggested working dilution to label 10^6 cells orcells or 100l human whole blood.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.1 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Flow Cytometry (FACS) image for anti-CD8 antibody (CD8a Molecule)  (FITC) (ABIN119507) Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD8:FITC (ABIN11...
Background publications Nelson, Lever, Dean et al.: "Characterization of lethal inhalational infection with Francisella tularensis in the common marmoset (Callithrix jacchus)." in: Journal of medical microbiology, Vol. 59, Issue Pt 9, pp. 1107-13, 2010 (PubMed).

Kap, van Meurs, van Driel et al.: "A monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 57, Issue 12, pp. 1159-67, 2009 (PubMed).

Gibbings, Marcet-Palacios, Sekar et al.: "CD8 alpha is expressed by human monocytes and enhances Fc gamma R-dependent responses." in: BMC immunology, Vol. 8, pp. 12, 2007 (PubMed).

Zarkesh-Esfahani, Pockley, Metcalfe et al.: "High-dose leptin activates human leukocytes via receptor expression on monocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 8, pp. 4593-9, 2001 (PubMed).

Parnes: "Molecular biology and function of CD4 and CD8." in: Advances in immunology, Vol. 44, pp. 265-311, 1989 (PubMed).