CD45 antibody

Catalog No. ABIN119550

Product Details anti-CD45 Antibody

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Reactivity: Human, Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD45 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: The antibody MEM-55 recognizes a siliadase-sensitive epitope of CD45RB, a 180-240 kDa single chain type I membrane glycoprotein, variant of CD45 (CD45RB isoform). CD45RB is expressed on a subset of T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells.

Cross-Reactivity (Details): Species reactivity (tested):Human, non-Human Primates.

Purification: Affinity Chromatography on Protein A

Immunogen: Human thymocytes and T lymphocytes

Clone: MEM-55

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry. Immunoprecipitation. Western Blotting. Immunohistochemistry (FFPE sections). No pre-treatment of tissue sections is essential.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH ~7.4, 15 mM Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Do not freeze working dilutions.

Storage: 4 °C/-20 °C

Storage Comment: Store the original vial at 2-8 °C. Freeze stock solution or aliquots thereof at -20 °C.

References for anti-CD45 antibody (ABIN119550)

de Torre-Minguela, Barberà-Cremades, Gómez, Martín-Sánchez, Pelegrín: "Macrophage activation and polarization modify P2X7 receptor secretome influencing the inflammatory process." in: Scientific reports, Vol. 6, pp. 22586, (2017) (PubMed).

Honda, Yasuda, Tanaka, Hagiwara, Arai, Yoshino: "Functional expression of full-length TrkA in the prokaryotic host Magnetospirillum magneticum AMB-1 by using a magnetosome display system." in: Applied and environmental microbiology, Vol. 81, Issue 4, pp. 1472-6, (2016) (PubMed).

Koethe, Zander, Köster, Annan, Ebenfelt, Spencer, Bemark: "Pivotal advance: CD45RB glycosylation is specifically regulated during human peripheral B cell differentiation." in: Journal of leukocyte biology, Vol. 90, Issue 1, pp. 5-19, (2011) (PubMed).

Möbius-Winkler, Hilberg, Menzel, Golla, Burman, Schuler, Adams: "Time-dependent mobilization of circulating progenitor cells during strenuous exercise in healthy individuals." in: Journal of applied physiology (Bethesda, Md. : 1985), Vol. 107, Issue 6, pp. 1943-50, (2010) (PubMed).

Curnis, Gasparri, Sacchi, Cattaneo, Magni, Corti: "Targeted delivery of IFNgamma to tumor vessels uncouples antitumor from counterregulatory mechanisms." in: Cancer research, Vol. 65, Issue 7, pp. 2906-13, (2005) (PubMed).

Reynaud, Bruno, Boullanger, Grange, Barbesti, Niveleau: "Monitoring of urinary excretion of modified nucleosides in cancer patients using a set of six monoclonal antibodies." in: Cancer letters, Vol. 61, Issue 3, pp. 255-62, (1992) (PubMed).

Target Details for CD45

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Alternative Name: CD45 / LCA (PTPRC Products)

Synonyms: PTPRC antibody, si:dkeyp-47c5.1 antibody, B220 antibody, CD45 antibody, CD45R antibody, GP180 antibody, L-CA antibody, LCA antibody, LY5 antibody, T200 antibody, Cd45 antibody, Ly-5 antibody, Lyt-4 antibody, loc antibody, Lca antibody, RT7 antibody, cd45 antibody, protein tyrosine phosphatase, receptor type C antibody, protein tyrosine phosphatase, receptor type, C antibody, receptor-type tyrosine-protein phosphatase C antibody, PTPRC antibody, ptprc antibody, LOC100074271 antibody, Ptprc antibody

Background: CD45RB is an of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RB is expressed e.g. in microglia and inflammatory cells.Synonyms: L-CA, Leukocyte common antigen, PTPRC, Receptor-type tyrosine-protein phosphatase C, T200

Gene ID: 5788

NCBI Accession: NP_002829

UniProt: P08575

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling