5-Methyl Cytosine (5-MeC) antibody

Antigen: 5-Methyl Cytosine (5-MeC)

Clonality: Monoclonal (33D3)

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN119697

Additional Information

Isotype: IgG1  (Matching secondary antibodies)

Clone: 33D3

Description: 5-methylcytidine (5-MeCyd) is found in DNA of plants and vertebrates. DNA methylation is a DNA modification process, which is involved in the control of gene expression. Reports suggest that in tumours, DNA is frequently globally hypomethylated compared to the DNA from normal tissue. Alternate Names: 5MeC.

Specificity: This antibody recognises the modified base 5-methylcytidine (5-MeCyd). Clone 33D3 has been developed to discriminate between the modified base 5-MeCyd and the normal counterpart cytosine.

Application Details

Application Notes: Flow cytometry. Immunoblotting. Immunofluorescence. Immunohistochemistry on frozen and paraffin embedded sections. This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. ABIN119697 has been reported for use in Methylated DNA immunoprecipitation (MeDIP). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Buffer: PBS, pH 7.4, containing 0.01% thimerosal as preservative

Storage: Store the antibody undiluted at 2-8°C for up to one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Restrictions: For Research Use only

Publications

Publications: Reynaud, Bruno, Boullanger et al.: "Monitoring of urinary excretion of modified nucleosides in cancer patients using a set of six monoclonal antibodies." in: Cancer letters, Vol. 61, Issue 3, pp. 255-62, 1992 (PubMed).

Habib, Fares, Bourgeois et al.: "DNA global hypomethylation in EBV-transformed interphase nuclei." in: Experimental cell research, Vol. 249, Issue 1, pp. 46-53, 1999 (PubMed).

Hernandez-Blazquez, Habib, Dumollard et al.: "Evaluation of global DNA hypomethylation in human colon cancer tissues by immunohistochemistry and image analysis." in: Gut, Vol. 47, Issue 5, pp. 689-93, 2000 (PubMed).