This antibody recognises an intracellular region of CD79b, a cell surface antigen expressed by B lymphocytes.
Purification
Affinity chromatography on Protein G
Immunogen
Peptide corresponding to an intracellular region of human CD79b. Spleen cells from immunised rat were fused with cells of the mouse NS-1 myeloma cell line.
Flow cytometry: 1/50 - 1/100, use 10 μL of the suggested working dilution to label 10^6 cells, Membrane permeabilisation is required for this application. Immunoprecipitation. ELISA. Western Blot. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
TRIS buffered saline pH 8.0 containing 0.09 % Sodium Azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
The CD79b molecule (also known as Ig beta and B29) associates with CD79a to form a heterodimer that is covalently linked to surface immunoglobulin, forming the B-cell receptor (BCR) complex.Synonyms: B-Cell marker, B-cell antigen receptor complex-associated protein beta-chain, B-cell-specific glycoprotein B29, B29, IGB, Immunoglobulin-associated B29 protein