Junctional Adhesion Molecule 2 (JAM2) antibody

Antigen: Junctional Adhesion Molecule 2 (JAM2)

Synonyms: JAMB, CD322, JAM-B, VEJAM, PRO245, VE-JAM, C21orf43, JAM-2, Jcam2, AU016127, 1110002N23Rik, 2410030G21Rik, 2410167M24Rik, JAM2, MGC151720, jamb, cd322, jam-b, vejam, pro245, ve-jam, MGC146002, sb:cb1002, sc:d0813, im:7155552, LOC100227766

Clonality: Monoclonal (CRAM-18 F26)

Host: Rat

Reactivity: Mouse (Murine), Human

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Functional Studies (Func)

Catalog no.: ABIN119731

Additional Information

Alternative name: CD322 / JAM2

Immunogen: Recombinant soluble JAM-2. Spleen cells from immunised Fischer rats were fused with cells of the Sp2/0 myeloma cell line

Isotype: IgG2a  (Matching secondary antibodies)

Clone: CRAM-18 F26

Description: JAM-2 is a 45kD transmembrane type 1 protein, a member of the immunoglobulin superfamily otherwise known as CD322. In adult murine tissue, CD322 expression is reported to be restricted to high endothelial venules of lymphoid organs, lymphoendothelial cells and endothelial cells in kidney. In humans, CD322 expression is not restricted to endothelial cells, but is also expressed on human lymphocytes.

Specificity: This antibody recognises junctional adhesion molecule-2 (JAM-2). Clone CRAM-18 F26 has been reported to inhibit transendothelial migration.

Synonyms: JAMB, CD322, JAM-B, VEJAM, PRO245, VE-JAM, C21orf43, JAM-2, Jcam2, AU016127, 1110002N23Rik, 2410030G21Rik, 2410167M24Rik, JAM2, MGC151720, jamb, cd322, jam-b, vejam, pro245, ve-jam, MGC146002, sb:cb1002, sc:d0813, im:7155552, LOC100227766

Application Details

Application Notes: Flow Cytometry: 1/10 - 1/25, Use 10ul of the suggested working dilution to label 10e6 cells in 100ul. Immunohistochemistry on frozen sections. Functional Assays. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Buffer: PBS, pH7.4

Storage: Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: Cell/Tissue Markers

Restrictions: For Research Use only

Publications

Publications: Aurrand-Lions, Duncan, Ballestrem et al.: "JAM-2, a novel immunoglobulin superfamily molecule, expressed by endothelial and lymphatic cells." in: The Journal of biological chemistry, Vol. 276, Issue 4, pp. 2733-41, 2001 (PubMed).

Aurrand-Lions, Johnson-Leger, Wong et al.: "Heterogeneity of endothelial junctions is reflected by differential expression and specific subcellular localization of the three JAM family members." in: Blood, Vol. 98, Issue 13, pp. 3699-707, 2001 (PubMed).