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Junctional Adhesion Molecule 2 (JAM2) antibody (Biotin)

Antigen

Junctional Adhesion Molecule 2 (JAM2)

Synonyms
JAMB, CD322, JAM-B, VEJAM, PRO245, VE-JAM, C21orf43, JAM-2, Jcam2, AU016127, 1110002N23Rik, 2410030G21Rik, 2410167M24Rik, JAM2, MGC151720, jamb, cd322, jam-b, vejam, pro245, ve-jam, MGC146002, sb:cb10 ... show more
Clonality Monoclonal (CRAM-19 H36)
Host
Reactivity
Alternatives

Mouse (Murine), Human

Conjugate
Alternatives Biotin
Application
Alternatives Flow Cytometry (FACS)
1 reference available
Catalog no. ABIN119745
Quantity 0.1 mg  (0.1 mg/ml)
Price 440.00 $   Plus shipping costs $35.00
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Availability Ships within 7 to 10 Business Days

Additional Information

Alternative name CD322 / JAM2
Immunogen Recombinant soluble JAM-2. Spleen cells from immunised Fischer rats were fused with cells of the Sp2/0 myeloma cell line
Isotype IgG2a
Clone CRAM-19 H36
Description JAM-2 is a 45kD transmembrane protein member of the immunoglobulin superfamily otherwise known as CD322. In adult murine tissue CD322 expression is reported to be restricted to high endothelial venules of lymphoid organs, lymphoendothelial cells and endothelial cells in kidney. In humans, CD322 expression is not restricted to endothelial cells, but is also expressed on human lymphocytes.
Specificity This antibody recognises junctional adhesion molecule-2 (JAM-2).
Synonyms JAMB, CD322, JAM-B, VEJAM, PRO245, VE-JAM, C21orf43, JAM-2, Jcam2, AU016127, 1110002N23Rik, 2410030G21Rik, 2410167M24Rik, JAM2, MGC151720, jamb, cd322, jam-b, vejam, pro245, ve-jam, MGC146002, sb:cb1002, sc:d0813, im:7155552, LOC100227766

Application Details

Application Notes Flow Cytometry: Neat - 1/5, Use 10ul of the suggested working dilution to label 10e6 cells in 100ul, The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Concentration 0.1 mg/ml
Buffer PBS, pH7.4 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Research Area Cell/Tissue Markers
Restrictions For Research Use only

Publications

Publications Aurrand-Lions, Duncan, Ballestrem et al.: "JAM-2, a novel immunoglobulin superfamily molecule, expressed by endothelial and lymphatic cells." in: The Journal of biological chemistry, Vol. 276, Issue 4, pp. 2733-41, 2001 (PubMed).

Alternatives

Alternatives for antigen "Junctional Adhesion Molecule 2 (JAM2)", type "Antibodies"
Hosts Rat (8), Goat (2), Mouse (1), Rabbit (1)
Reactivities Human (11), Mouse (Murine) (10), Cow (Bovine) (1), Dog (Canine) (1), Rat (Rattus) (1)
Applications Flow Cytometry (FACS) (8), Immunohistochemistry (Frozen Sections) (IHC (fro)) (5), Western Blotting (WB) (4), Enzyme Immunoassay (EIA) (2), Immunoprecipitation (IP) (2), ELISA (1), Functional Studies (Func) (1), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (1)
Conjugates FITC (2), Biotin (1)