Junctional Adhesion Molecule 2 (JAM2) antibody (FITC)

Antigen: Junctional Adhesion Molecule 2 (JAM2)

Synonyms: JAMB, CD322, JAM-B, VEJAM, PRO245, VE-JAM, C21orf43, JAM-2, Jcam2, AU016127, 1110002N23Rik, 2410030G21Rik, 2410167M24Rik, JAM2, MGC151720, jamb, cd322, jam-b, vejam, pro245, ve-jam, MGC146002, sb:cb1002, sc:d0813, im:7155552, LOC100227766

Clonality: Monoclonal (CRAM-19 H36)

Host: Rat

Reactivity: Mouse (Murine), Human

Conjugate: FITC

Application: Flow Cytometry (FACS)

Catalog no.: ABIN119746

Additional Information

Alternative name: CD322 / JAM2

Immunogen: Recombinant soluble JAM-2. Spleen cells from immunised Fischer rats were fused with cells of the Sp2/0 myeloma cell line

Isotype: IgG2a

Clone: CRAM-19 H36

Description: JAM-2 is a 45kD transmembrane protein, a member of the immunoglobulin superfamily otherwise known as CD322. In adult murine tissue CD322 expression is reported to be restricted to high endothelial venules of lymphoid organs, lymphoendothelial cells and endothelial cells in kidney. In humans, CD322 expression is not restricted to endothelial cells, but is also expressed on human lymphocytes.

Specificity: This antibody recognises junctional adhesion molecule-2 (JAM-2).

Synonyms: JAMB, CD322, JAM-B, VEJAM, PRO245, VE-JAM, C21orf43, JAM-2, Jcam2, AU016127, 1110002N23Rik, 2410030G21Rik, 2410167M24Rik, JAM2, MGC151720, jamb, cd322, jam-b, vejam, pro245, ve-jam, MGC146002, sb:cb1002, sc:d0813, im:7155552, LOC100227766

Application Details

Application Notes: Flow Cytometry: Neat - 1/10, Use 10ul of the suggested working dilution to label 10e6 cells in 100ul, The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.1 mg/ml

Buffer: PBS, pH7.4 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light. Shelf Life: one year from despatch.

Research Area: Cell/Tissue Markers

Restrictions: For Research Use only

Publications

Publications: Aurrand-Lions, Duncan, Ballestrem et al.: "JAM-2, a novel immunoglobulin superfamily molecule, expressed by endothelial and lymphatic cells." in: The Journal of biological chemistry, Vol. 276, Issue 4, pp. 2733-41, 2001 (PubMed).