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CD47 antibody

CD47 Reactivity: Rat FACS, WB, IP Host: Mouse Monoclonal OX101 unconjugated

Catalog No. ABIN119763

Datasheet as PDF

Target See all CD47 Antibodies

CD47
Reactivity
120

45

27

6

4

2

2

2

1

1

1

1
Rat
Host
79

62

1

1
Mouse
Compatible Secondaries
Clonality
74

67

1
Monoclonal
Conjugate
64

12

11

5

5

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1
This CD47 antibody is un-conjugated
Application
82

81

43

41

30

26

26

18

16

14

13

11

7

5

4

3

3

2

2

2

1

1
Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP)

Specificity

This antibody recognises the homologue of human CD47, which has been identified as the ligand for the signal regulatory protein (SIRP). Clone OX-101 has been reported to block the interaction of CD47 and SIRP. We recommend the use of SM2002A for this purpose.

Purification

Affinity chromatography on Protein G

Immunogen

Rat thymocytes.

Clone

OX101

Isotype

IgG1

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anti-CD47 (CD47) (AA 18-135) antibody
CD47 Reactivity: Human FACS, WB, IF, IHC, ELISA, StM, Coat Host: Mouse Monoclonal CD47-2937 unconjugated

anti-CD47 (CD47) antibody
CD47 Reactivity: Human, Pig, Non-Human Primate FACS, WB, IHC (fro) Host: Mouse Monoclonal MEM-122 unconjugated

anti-CD47 (CD47) antibody
CD47 Reactivity: Human, Mouse, Rat IF, IHC Host: Rabbit Polyclonal unconjugated

anti-CD47 (CD47) antibody
CD47 Reactivity: Human WB, IF, IHC, ELISA Host: Rabbit Monoclonal 4G11 unconjugated Recombinant Antibody

anti-CD47 (CD47) (Internal Region) antibody
CD47 Reactivity: Human WB, ELISA Host: Rabbit Polyclonal unconjugated

Application Notes

Flow Cytometry: 1/10 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions

For Research Use only
Concentration

1.0 mg/mL

Buffer

PBS containing 0.09 % Sodium Azide

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeated freezing and thawing.

Storage

4 °C/-20 °C

Storage Comment

Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target

CD47

Alternative Name

CD47 (CD47 Products)

Synonyms

IAP antibody, MER6 antibody, OA3 antibody, 9130415E20Rik antibody, AA407862 antibody, AI848868 antibody, AW108519 antibody, B430305P08Rik antibody, Itgp antibody, CD47/IAP antibody, alkaline phosphatase, intestinal antibody, CD47 molecule antibody, CD47 antigen (Rh-related antigen, integrin-associated signal transducer) antibody, Cd47 molecule antibody, ALPI antibody, CD47 antibody, Cd47 antibody

Background

Rat CD47 is expressed primarily by lymphocytes and macrophages but has also been detected at high levels on brain endothelial cells.Synonyms: Antigenic surface determinant protein OA3, IAP, Integrin-associated protein, Leukocyte surface antigen CD47, MER6, OA3

Gene ID

29364

NCBI Accession

NP_062068

UniProt

P97829