This antibody recognises human CD177 (neutrophil glycoprotein NB1). The neutrophil NB1 antigen is expressed by 97 % of the caucasian population. Antibodies against NB1 can be involved in neonatal alloimmune neutropenia (NAN). Antibody reactivity and working conditions may vary between species.
Flow cytometry (use 10 μL of neat - 1/10 diluted antibody to label 10^6 cells in 100 μL). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.1 mg/mL
Buffer
PBS with 0.09 % sodium azide as preservative and 1 % BSA as stabelizer
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody at 2-8 °C for one month or (in small aliquots) at -20 °C for longer.
Cd177 (NP1), a glycosyl phosphatidylinositol (GPI) linked N glycosylated cell surface glycoprotein, was first described in a case of neonatal alloimmune neutropenia and analyses showed cell surface expression of a 60 kDa protein, 14 kDa greater than the predicted size, probably due to the presence of 3 potential N glycosylation sites. Immunohistochemistry demonstrated expression in bone marrow early erythroblasts, megakaryocytes, promyelocytes, and myelocytes. The neutrophil NB1 antigen (CD177) is expressed by 97 % of the caucasian population. Antibodies against NB1 can be involved in neonatal alloimmune neutropenia (NAN).Synonyms: HNA-2a, HNA2a, Human neutrophil alloantigen 2a, NB1, NB1 glycoprotein, PRV1, Polycythemia rubra vera protein 1