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ITGA5 antibody (Integrin, alpha 5 (Fibronectin Receptor, alpha Polypeptide)) (FITC)

Details for Product anti-ITGA5 Antibody No. ABIN119996, Supplier: Log in to see
Antigen
  • CD49e
  • Cd49e
  • FNRA
  • Fnra
  • ITGA5
  • Itga5_retired
  • VLA5
  • VLA5A
Reactivity
Mouse (Murine)
240
159
42
3
3
3
3
1
Host
Rat
129
117
80
31
4
Clonality (Clone)
Monoclonal ()
Conjugate
This ITGA5 antibody is conjugated to FITC
39
27
23
8
8
7
6
4
3
3
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
211
139
102
83
58
54
21
18
16
11
6
6
3
2
2
2
2
1
1
1
Supplier
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Immunogen Mouse MC/9 cell line. Remarks: Spleen cells from immunised Lewis rats were fused with cells of the Murine P3X63Ag8.653
Clone MFR5 (5H10)
Isotype IgG2a
Cross-Reactivity (Details) Species reactivity (tested):Mouse.
Purification Affinity Chromatography on Protein G
Alternative Name CD49e / ITGA5 (ITGA5 Antibody Abstract)
Background Integrin alpha 5 belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post translational cleavage in the extracellular domain to yield disulfide linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell surface mediated signalling. It is expressed on thymocytes, activated T cells, mast cells, and a variety of mouse cell lines.Synonyms: CD49 antigen-like family member E, FNRA, Fibronectin receptor subunit alpha, Integrin alpha-5, Integrin alpha-F, VLA-5, VLA5
Gene ID 10090
UniProt P11688
Research Area CD Antigens, Surface Receptors of Immune Cells
Application Notes Flow Cytometry: The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.1 mg/mL
Buffer PBS containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Flow Cytometry (FACS) image for anti-ITGA5 antibody (Integrin, alpha 5 (Fibronectin Receptor, alpha Polypeptide))  (FITC) (ABIN119996) Staining of mouse peripheral blood lymphocytes with RAT ANTI MOUSE CD49e: FITC (ABIN1...
Background publications Cukierman, Pankov, Stevens, Yamada: "Taking cell-matrix adhesions to the third dimension." in: Science (New York, N.Y.), Vol. 294, Issue 5547, pp. 1708-12, 2001

van der Loo, Xiao, McMillin, Hashino, Kato, Williams: "VLA-5 is expressed by mouse and human long-term repopulating hematopoietic cells and mediates adhesion to extracellular matrix protein fibronectin." in: The Journal of clinical investigation, Vol. 102, Issue 5, pp. 1051-61, 1998

Kinashi, Springer: "Adhesion molecules in hematopoietic cells." in: Blood cells, Vol. 20, Issue 1, pp. 25-44, 1995