Integrin, alpha 5 (Fibronectin Receptor, alpha Polypeptide) (ITGA5) antibody (FITC)
| Antigen |
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| Synonyms |
CD11C, SLEB6, Cr4, N418, Cd11c, AI449405, ITGAX, ITGA5, CD11c |
| Clonality |
Monoclonal (MFR5 (5H10)) |
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Host
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Reactivity
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Conjugate
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FITC (27), Biotin (14), PE (12), RPE (2), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
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Application
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Flow Cytometry (FACS) (101), Western Blotting (WB) (35), ELISA (25), Immunoprecipitation (IP) (24), Immunofluorescence (IF) (23), Immunohistochemistry (Fixed) (IHC (fx)) (20), Immunohistochemistry (Frozen Sections) (IHC (fro)) (15), Functional Studies (Func) (14), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (8), Immunohistochemistry (IHC) (7), Immunocytochemistry (ICC) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Blocking Antibody (Inhibition) (1), Immunoelectron Microscopy (IEM) (1), Radioimmunoassay (RIA) (1)
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| Catalog no. |
ABIN119997 |
| Quantity |
0.5 mg (0.5 mg/ml) (Variants) |
| Price |
506.00 $ Plus shipping costs $45.00
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| Shipping to |
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| Availability |
Will be delivered in 6 to 8 Business Days |
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Alternative name
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CD49e / ITGA5
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UniProt
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P11688
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Immunogen
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Mouse MC/9 cell line. Spleen cells from immunised Lewis rats were fused with cells of the Murine P3X63Ag8.653
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Cross-Reactivity
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Mouse (Murine)
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Isotype
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IgG2a
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Clone
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MFR5 (5H10)
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Description
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VLA-5 plays an important role in cell adhesion and migration and is expressed by mast cells, thymocytes, Tcells and monocytes. Alternate Names: VLA-5, INTEGRIN ALPHA 5 CHAIN.
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Application Notes
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Flow Cytometry: 1/10, Use 10ul of the suggested working dilution to label 10e6 cells in 100ml, The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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0.5 mg/ml
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Buffer
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PBS, pH7.4 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin
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Storage
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Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light. Shelf Life: one year from despatch.
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Research Area
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CD Antigens, Surface Receptors of Immune Cells
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Restrictions
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For Research Use only
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Kinashi, Springer: "Adhesion molecules in hematopoietic cells." in: Blood cells, Vol. 20, Issue 1, pp. 25-44, 1995 (PubMed).
Cukierman, Pankov, Stevens et al.: "Taking cell-matrix adhesions to the third dimension." in: Science (New York, N.Y.), Vol. 294, Issue 5547, pp. 1708-12, 2001 (PubMed).
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Alternatives for antigen "Integrin, alpha 5 (Fibronectin Receptor, alpha Polypeptide) (ITGA5)", type "Antibodies"
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Hosts
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Mouse (39), Rabbit (38), Rat (36), Hamster (34)
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Reactivities
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Mouse (Murine) (92), Human (76), Rat (Rattus) (35), Cow (Bovine) (20), Horse (Equine) (18), Pig (Porcine) (18), Dog (Canine) (3), Cat (Feline) (1), Chicken (1)
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Applications
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Flow Cytometry (FACS) (101), Western Blotting (WB) (35), ELISA (25), Immunoprecipitation (IP) (24), Immunofluorescence (IF) (23), Immunohistochemistry (Fixed) (IHC (fx)) (20), Immunohistochemistry (Frozen Sections) (IHC (fro)) (15), Functional Studies (Func) (14), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (8), Immunohistochemistry (IHC) (7), Immunocytochemistry (ICC) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Blocking Antibody (Inhibition) (1), Immunoelectron Microscopy (IEM) (1), Radioimmunoassay (RIA) (1)
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Conjugates
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FITC (27), Biotin (14), PE (12), RPE (2), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
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Epitopes
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C-Term (9), Center (2), Heavy Chain, Cleaved-Phe42 (1), Light Chain, Cleaved-Glu895 (1), N-Term (1)
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