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Integrin, alpha 5 (Fibronectin Receptor, alpha Polypeptide) (ITGA5) antibody

Details for Product No. ABIN119998, Supplier: Log in to see
Antigen
  • ITGA5
  • Cd49e
  • Fnra
  • VLA5
  • CD49e
  • FNRA
  • VLA5A
  • Itga5_retired
Reactivity
Mouse (Murine)
190
159
42
3
3
3
3
1
Host
Rat
117
80
79
31
4
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
38
25
20
6
5
5
5
4
1
1
1
1
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
198
121
70
70
44
41
21
16
16
11
6
6
3
2
2
2
2
1
1
1
Supplier
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Immunogen Mouse MC/9 cell line. Spleen cells from immunised Lewis rats were fused with cells of the Murine P3X63Ag8.653
Clone MFR5 (5H10)
Isotype IgG2a
Purification Affinity chromatography on Protein G
Endotoxin Level Low Endotoxin
Alternative Name CD49e / ITGA5 (ITGA5 Antibody Abstract)
Background VLA-5 plays an important role in cell adhesion and migration and is expressed by mast cells, thymocytes, Tcells and monocytes.Synonyms: CD49 antigen-like family member E, FNRA, Fibronectin receptor subunit alpha, Integrin alpha-5, Integrin alpha-F, VLA-5, VLA5
Gene ID 10090
UniProt P11688
Research Area CD Antigens, Surface Receptors of Immune Cells
Application Notes Flow Cytometry: 1/10 - 1/25, Use 10 μL of the suggested working dilution to label 10^6 cells in 100ml. Immunohistochemistry on frozen sections. Functional Assays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level: less than 0.01 EU/ug
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody at -20 °C. Ship at 2 - 8 °C.
Background publications Cukierman, Pankov, Stevens et al.: "Taking cell-matrix adhesions to the third dimension." in: Science (New York, N.Y.), Vol. 294, Issue 5547, pp. 1708-12, 2001 (PubMed).

Kinashi, Springer: "Adhesion molecules in hematopoietic cells." in: Blood cells, Vol. 20, Issue 1, pp. 25-44, 1995 (PubMed).