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Integrin, alpha 5 (Fibronectin Receptor, alpha Polypeptide) (ITGA5) antibody

Details for Product No. ABIN120000, Supplier: Log in to see
Antigen
  • ITGA5
  • Cd49e
  • Fnra
  • VLA5
  • CD49e
  • FNRA
  • VLA5A
  • Itga5_retired
Reactivity
Mouse (Murine)
190
159
42
3
3
3
3
1
Host
Rat
117
80
79
31
4
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
30
20
17
6
5
5
5
4
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
198
121
70
70
44
41
21
17
16
11
6
6
3
2
2
2
2
1
1
1
Supplier
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Immunogen Mouse MC/9 cell line. Spleen cells from immunised Lewis rats were fused with cells of the Murine P3X63Ag8.653
Clone MFR5 (5H10)
Isotype IgG2a
Purification Affinity Chromatography on Protein G.
Alternative Name CD49e / ITGA5 (ITGA5 Antibody Abstract)
Background VLA-5 plays an important role in cell adhesion and migration and is expressed by mast cells, thymocytes, Tcells and monocytes.Synonyms: CD49 antigen-like family member E, FNRA, Fibronectin receptor subunit alpha, Integrin alpha-5, Integrin alpha-F, VLA-5, VLA5
Gene ID 10090
UniProt P11688
Research Area CD Antigens, Surface Receptors of Immune Cells
Application Notes Flow Cytometry (1/10-1/25): Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemitry on Frozen Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Flow Cytometry (FACS) image for anti-Integrin, alpha 5 (Fibronectin Receptor, alpha Polypeptide) (ITGA5) antibody (ABIN120000) Staining of mouse peripheral blood lymphocytes with RAT ANTI MOUSE CD49e (ABIN120000).
Background publications Cukierman, Pankov, Stevens et al.: "Taking cell-matrix adhesions to the third dimension." in: Science (New York, N.Y.), Vol. 294, Issue 5547, pp. 1708-12, 2001 (PubMed).

Kinashi, Springer: "Adhesion molecules in hematopoietic cells." in: Blood cells, Vol. 20, Issue 1, pp. 25-44, 1995 (PubMed).