Carbohydrate Antigen 19-9 (CA19-9) antibody

Antigen: Carbohydrate Antigen 19-9 (CA19-9)

Clonality: Monoclonal (1116-NS-19-9)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN120086

Additional Information

Alternative name: CA 19-9

Immunogen: SW1116 human colorectal cancer cells

Isotype: IgG1

Clone: 1116-NS-19-9

Description: In malignant tissues positive staining of tumour cells is observed in most adenocarcinomas of the pancreas and stomach, and in a large proportion of colon and gall bladder tumours. The antigen is also expressed in approximately half of primary and metastatic ovarian tumours. In normal tissues positive staining is also observed in columnar epithelium of the pancreas stomach, liver, gall bladder and in bronchial glands of the lung.

Specificity: This antibody is a carbohydrate antigenic determinant identified as a sialylated lacto-N- fucopentose II, an oligosaccharide biochemically related to the Lewis A blood group antigen.

Application Details

Application Notes: Immunohistochemistry on frozen and paraffin sections: 1/10 - 1/40, this product does not require protein digestion pre-treatment of paraffin sections, this product does not require antigen retrieval using heat treatment prior to staining of paraffin sections, A minimum incubation time of 1 hour is recommended. Recommended positive control: Adenocarcinoma of colon. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Buffer: PBS containing 0.09% Sodium Azide and 1% Bovine Serum Albumin

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: Cancer, Cell/Tissue Markers, Blood Components

Restrictions: For Research Use only