ITGB1 antibody (FITC)

Catalog No. ABIN120156

Product Details anti-ITGB1 Antibody (FITC)

Target: ITGB1 (Integrin beta 1 (ITGB1))

Reactivity: Mouse, Rat

Host: Hamster

Clonality: Monoclonal

Conjugate: This ITGB1 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Specificity: This antibody recognises the integrin beta 1 subunit (CD29). Clone HM beta 1.1 is reported to inhibit beta 1 integrin mediated adhesion. We recommend the use of SM2210LE for this purpose.

Purification: Affinity Chromatography on Protein G.

Immunogen: Purified mouse VLA-4 antigen. Spleen cells from immunised Armenian hamsters were fused with cells of the P3U1 mouse myeloma cell line.

Clone: HM beta 1-1

Isotype: IgG

Application Details

Application Notes: Flow Cytometry (Neat-1/10): Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ITGB1

Target: ITGB1 (Integrin beta 1 (ITGB1))

Alternative Name: CD29 / Integrin beta-1 (ITGB1 Products)

Synonyms: CD29 antibody, FNRB antibody, GPIIA antibody, MDF2 antibody, MSK12 antibody, VLA-BETA antibody, VLAB antibody, 4633401G24Rik antibody, AA409975 antibody, AA960159 antibody, ENSMUSG00000051907 antibody, Fnrb antibody, Gm9863 antibody, gpIIa antibody, cd29 antibody, fnrb antibody, gpiia antibody, itgb1 antibody, mdf2 antibody, msk12 antibody, vla-beta antibody, vlab antibody, itgb1-a antibody, itgb1-b antibody, integrin subunit beta 1 antibody, integrin beta 1 (fibronectin receptor beta) antibody, integrin subunit beta 1 S homeolog antibody, integrin beta 1 antibody, integrin subunit beta 1 L homeolog antibody, ITGB1 antibody, Itgb1 antibody, itgb1.S antibody, LOC100009173 antibody, itgb1.L antibody

Background: CD29 is a 110KD cell surface glycoprotein that is widely expressed by a variety of cells including all leucocytes. CD29 forms non-covalent bonds with the integrin alpha subunits, including CD51 and CD49a-f, to form heterodimers. The ligands for these heterodimers include collagen, fibronectin, laminin and vascular adhesion molecule-1. In the immune system beta 1 integrins play an important role in cell adhesion, migration, activation and differentiation.Synonyms: FNRB, Fibronectin receptor subunit beta, ITGB1, Integrin VLA-4 subunit beta, MDF2, MSK12

Gene ID: 16412

NCBI Accession: NP_034708

UniProt: P09055

Pathways: Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor, Integrin Complex, SARS-CoV-2 Protein Interactome