EMR2 antibody

Catalog No. ABIN120169

Product Details anti-EMR2 Antibody

Target: EMR2 (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 2 (EMR2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EMR2 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody recognises EMR2, a member of the epidermal growth factor-seven transmembrane (EGFTM7) family of proteins, which is closely related to CD97. Clone 2A1 specifically recognises the stalk region of EMR2.

Purification: Affinity Chromatography on Protein G.

Immunogen: NIH-3T3 cells stably transfected with EMR2 (EGF1-5) cDNA. Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.

Clone: 2A1

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: Use 10 μL of 1/50-1/200 diluetd antibody to label 10^6 cells in 100 μL. Immunoprecipitation. Western Blot. Immunohistochemistry on Frozen Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for EMR2

Target: EMR2 (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 2 (EMR2))

Alternative Name: CD312 / EMR2 (EMR2 Products)

Synonyms: EMR2 antibody, CD312 antibody, adhesion G protein-coupled receptor E2 antibody, ADGRE2 antibody, LOC484898 antibody

Background: EMR2 is a member of the epidermal growth factor-seven transmembrane (EGF-TM7) family of proteins, which is closely related to CD97. EMR2, also known as CD312, is predominantly expressed on myeloid dendritic cells, monocytes and tissue macrophages. Various isoforms of EMR2 have been documented. The ligand for the largest isoform of EMR2 has recently been identified as chrondroitin sulphate, which binds to the fourth EGF-like module of EMR2.Synonyms: EGF-like module EMR2, EGF-like module-containing mucin-like hormone receptor-like 2

Gene ID: 30817

NCBI Accession: NP_038475

UniProt: Q9UHX3