Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 (CYP19A1) (AA 376-390) antibody

Details for Product No. ABIN120192, Supplier: Log in to see
Antigen
  • CYP19
  • CYP19A1
  • ARO
  • ARO1
  • CPV1
  • CYAR
  • CYPXIX
  • P-450AROM
  • Aromatase
  • Cyp19
  • Cyp19a
  • p450arom
  • CYP19P1
  • Ar
  • ArKO
  • Int-5
  • Int5
  • P450AROM
  • P450 arom
  • aro
  • cyp19
  • cyp19a
  • p450arom-A
  • aromatase
  • cytochrome P450 aromatase
  • cytochrome P450 19A1
  • cytochrome P450 19A3
  • cytochrome P450, family 17, subfamily A, polypeptide 1
  • cytochrome P450, family 19
  • cytochrome P450, family 19, subfamily A, polypeptide 1
  • cytochrome P450, family 19, subfamily a, polypeptide 1
  • SAV_2383
  • pfcyp19b-tstr
  • CYP19A1
  • CYP19A3
  • CYP19
  • Cyp19a1
  • cyp19a1
Alternatives
anti-Baboon Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 antibody for Immunofluorescence
Epitope
AA 376-390
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18
17
16
9
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5
5
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3
2
2
2
2
2
2
1
1
1
1
1
Reactivity
Baboon, Chicken, Cow (Bovine), Human, Marmoset, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)
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67
47
23
13
11
9
9
9
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3
3
1
1
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Host
Mouse
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30
20
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
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5
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2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
120
41
39
39
26
24
14
14
5
4
2
1
Options
Supplier
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Supplier Product No.
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Immunogen Synthetic peptide corresponding to amino acids 376-390 of Human Aromatase.
Clone H4
Isotype IgG2a
Specificity SM2222P recognises a conserved epitope within Cytochrome P450 Aromatase (P450 arom).
Purification Supernatant
Alternative Name Aromatase (CYP19A1 Antibody Abstract)
Background Aromatase is a key enzyme in steroidogenesis and plays an important role in sexual differentiation, oestrogen biosynthesis, fertility and carcinogenesis. It is highly conserved amongst mammals, and is highly expressed in placental tissue. Many environmental chemicals may influence aromatase activity and thereby disrupt endocrine function.Synonyms: ARO1, CYAR, CYP19, CYP19A1, CYPXIX, Cytochrome P450 19A1, Estrogen synthetase, P450AROM
Gene ID 1588
NCBI Accession NP_000094
UniProt P11511
Research Area Cancer, Endocrine system, Enzymes
Pathways Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process, Carbohydrate Homeostasis
Application Notes Immunohistochemistry on Paraffin Sections: does not require antigen retrieval using heat treatment. Recommended positive control tissue: human placenta. Western Blot (1/250): detects a band of approximately 55 kD in human placental extracts. P450 arom is highly expressed in placental tissue. For tissues where there may be low expression of P450 arom, the use of microsomal extracts may improve the staining for Western blots using this antibody (see ref. 1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Western Blotting (WB) image for anti-Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 (CYP19A1) (AA 376-390) antibody (ABIN120192) Western blot analysis of human placenta extract probed with antiI Hu Cytochrome P450 ...
Product cited in: Schicht, Ernst, Nielitz, Fester, Tsokos, Guddat, Bräuer, Bechmann, Delank, Wohlrab, Paulsen, Claassen: "Articular cartilage chondrocytes express aromatase and use enzymes involved in estrogen metabolism." in: Arthritis research & therapy, Vol. 16, Issue 2, pp. R93, 2014 (PubMed).

Horling, Santos, Fischer: "The AhR is constitutively activated and affects granulosa cell features in the human cell line KGN." in: Molecular human reproduction, Vol. 17, Issue 2, pp. 104-14, 2011 (PubMed).

Bender, Zhou, Wilkars, Fester, Lanowski, Paysen, König, Rune: "Roles of 17ß-estradiol involve regulation of reelin expression and synaptogenesis in the dentate gyrus." in: Cerebral cortex (New York, N.Y. : 1991), Vol. 20, Issue 12, pp. 2985-95, 2010 (PubMed).

Colette, Lousse, Defrère, Curaba, Heilier, Van Langendonckt, Mestdagt, Foidart, Loumaye, Donnez: "Absence of aromatase protein and mRNA expression in endometriosis." in: Human reproduction (Oxford, England), Vol. 24, Issue 9, pp. 2133-41, 2009 (PubMed).

Background publications Wu, Bennett, Talla, Stocco: "Testosterone, not 5α-dihydrotestosterone, stimulates LRH-1 leading to FSH-independent expression of Cyp19 and P450scc in granulosa cells." in: Molecular endocrinology (Baltimore, Md.), Vol. 25, Issue 4, pp. 656-68, 2011 (PubMed).

Jeong, Jung, Kim, Jin, Kim, Kang, Kim, van Wijnen, Stein, Lian, Stein, Kato, Choi: "The gene for aromatase, a rate-limiting enzyme for local estrogen biosynthesis, is a downstream target gene of Runx2 in skeletal tissues." in: Molecular and cellular biology, Vol. 30, Issue 10, pp. 2365-75, 2010 (PubMed).

Zhao, McBride, Nandi, McQueen, McGrew, Hocking, Lewis, Sang, Clinton: "Somatic sex identity is cell autonomous in the chicken." in: Nature, Vol. 464, Issue 7286, pp. 237-42, 2010 (PubMed).

Catalano, Malivindi, Giordano, Gu, Panza, Bonofiglio, Lanzino, Sisci, Panno, Andò: "Farnesoid X receptor, through the binding with steroidogenic factor 1-responsive element, inhibits aromatase expression in tumor Leydig cells." in: The Journal of biological chemistry, Vol. 285, Issue 8, pp. 5581-93, 2010 (PubMed).

"Retraction." in: Molecular endocrinology (Baltimore, Md.), 2010 (PubMed).

Lu, Kang, Hu: "BRCA1/BARD1 complex interacts with steroidogenic factor 1--A potential mechanism for regulation of aromatase expression by BRCA1." in: The Journal of steroid biochemistry and molecular biology, Vol. 123, Issue 1-2, pp. 71-8, 2010 (PubMed).

Ghosh, Lu, Hu: "A Role of CREB in BRCA1 Constitutive Promoter Activity and Aromatase Basal Expression." in: International journal of biomedical science : IJBS, Vol. 4, Issue 4, pp. 260-265, 2010 (PubMed).

Sirianni, Chimento, De Luca, Zolea, Carpino, Rago, Maggiolini, Andò, Pezzi: "Inhibition of cyclooxygenase-2 down-regulates aromatase activity and decreases proliferation of Leydig tumor cells." in: The Journal of biological chemistry, Vol. 284, Issue 42, pp. 28905-16, 2009 (PubMed).

Barone, Cui, Herynk, Corona-Rodriguez, Giordano, Selever, Beyer, Andò, Fuqua: "Expression of the K303R estrogen receptor-alpha breast cancer mutation induces resistance to an aromatase inhibitor via addiction to the PI3K/Akt kinase pathway." in: Cancer research, Vol. 69, Issue 11, pp. 4724-32, 2009 (PubMed).

Lu, Amleh, Sun, Jin, McCullough, Baer, Ren, Li, Hu: "Ubiquitination and proteasome-mediated degradation of BRCA1 and BARD1 during steroidogenesis in human ovarian granulosa cells." in: Molecular endocrinology (Baltimore, Md.), Vol. 21, Issue 3, pp. 651-63, 2007 (PubMed).

Carpino, Rago, Pezzi, Carani, Andò: "Detection of aromatase and estrogen receptors (ERalpha, ERbeta1, ERbeta2) in human Leydig cell tumor." in: European journal of endocrinology / European Federation of Endocrine Societies, Vol. 157, Issue 2, pp. 239-44, 2007 (PubMed).

Turner, Macpherson, Millar, McNeilly, Williams, Cranfield, Groome, Sharpe, Fraser, Saunders: "Development and validation of a new monoclonal antibody to mammalian aromatase." in: The Journal of endocrinology, Vol. 172, Issue 1, pp. 21-30, 2002 (PubMed).

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