Integrin beta 3 antibody (FITC)

Catalog No. ABIN120248

Product Details anti-Integrin beta 3 Antibody (FITC)

Target: Integrin beta 3 (ITGB3)

Reactivity: Mouse, Rat

Host: Hamster

Clonality: Monoclonal

Conjugate: This Integrin beta 3 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Purification: Affinity Chromatography on Protein G.

Immunogen: Mouse alpha 5 beta 3 protein purified from the mouse hybridoma 2B4. Spleen cells from immunised Armenian hamsters were fused with cells of the P3U1 mouse myeloma cell line.

Clone: HM beta 3-1

Isotype: IgG

Application Details

Application Notes: Flow Cytometry (Neat-1/10): Use 10 μL of of the suggested working dilution to label 10^6 cells in 100 μL. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Integrin beta 3

Target: Integrin beta 3 (ITGB3)

Alternative Name: CD61 / ITGB3 (ITGB3 Products)

Synonyms: BDPLT16 antibody, BDPLT2 antibody, CD61 antibody, GP3A antibody, GPIIIa antibody, GT antibody, INGRB3 antibody, integrin antibody, INTB3 antibody, GPIIIA antibody, cd61 antibody, gp3a antibody, itgb3 antibody, itgb3.2 antibody, itgb3.1 antibody, integrin subunit beta 3 antibody, integrin beta 3 antibody, integrin subunit beta 3 L homeolog antibody, integrin beta 3a antibody, integrin beta 3b antibody, ITGB3 antibody, Itgb3 antibody, itgb3.L antibody, itgb3a antibody, itgb3b antibody

Background: CD61 is a 90kD a type I membrane protein, which is expressed primarily on megakaryocytes, platelets, monocytes, macrophages, granulocytes and endothelial cells. CD61 associates with either the alpha IIb integrin (CD41) or the alpha V integrin (CD51) to form the platelet glycoprotein complex IIb/IIIa and the vitronectin receptor (VNR) respectively. The heterodimers formed with CD61 are receptors for a variety of ligands including fibrinogen, fibronectin, von Willebrands factor (vWF), vitronectin and thrombospondin.Synonyms: GP3A, GPIIIa, Integrin beta-3, Platelet membrane glycoprotein IIIa

Gene ID: 10090

UniProt: O54890

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Integrin Complex