Search, Find and Buy Antibodies, ELISA Kits and Proteins.
Loading Loading
Order hotline:
phone +1 404 474 4654
fax +1 888 205 9894 (TF)
Details for Product No. ABIN120290

Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1) antibody

Request
Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms CD314, D12S2489E, KLR, NKG2-D, NKG2D, Nkrp2, KLRK1, Nkg2d, D6H12S2489E
Reactivity
»Alternatives Human
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Functional Studies (Func), Immunoprecipitation (IP), Flow Cytometry (FACS)
Pubmed 3 references available
Catalog no. ABIN120290
Quantity 0.5 mg
Price
632.50 $   Plus shipping costs $45.00
Shipping to
Availability Will be delivered in 6 to 8 Business Days
Immunogen NKL cells. Spleen cells from immunised RBF/DnJ mice were fused with cells of the p3 mouse myeloma cell line.
Clone 5C6
Isotype IgG2a
Specificity This antibody is specific for CD314, also known as natural killer receptor G2 (NKG2D) and as killer cell lectin-like receptor subfamily K, member 1 (KLRK1). Clone 5C6 is reported to inhibit T cell recognition of MICA (1, 3).
Purification Affinity chromatography on Protein G
Endotoxin Level Low Endotoxin
Alternative Name CD314 / KLRK1
Background CD314 is a C-type lectin-like activating receptor which is expressed on most natural killer (NK) cells, CD8 T cells and gamma delta T cells. CD314 forms homodimers that signal through an associated DAP10 adaptor protein. Ligands of CD314 include MICA, MICB and UL16 binding protein (ULBP), which are inducibly expressed. Ligand binding to CD314 results in NK cell activation and potent costimulation of effector T cells.
Alternate names: D12S2489E, Killer cell lectin-like receptor subfamily K member 1, NK cell receptor D, NKG2-D type II integral membrane protein, NKG2-D-activating NK receptor, NKG2D
Gene ID 100528032
NCBI Accession NP_001186734.1
UniProt P26718
Application Notes Flow Cytometry: 1/100 - 1/200, use 10 ul of the suggested working dilution to label 106 cells in 100 ul. Immunoprecipitation. Functional assays. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level: less than 0.01 EU/mg
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS, pH7.4
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Bauer, Groh, Wu et al.: "Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA." in: Science (New York, N.Y.), Vol. 285, Issue 5428, pp. 727-9, 1999 (PubMed).

Wu, Song, Bakker et al.: "An activating immunoreceptor complex formed by NKG2D and DAP10." in: Science (New York, N.Y.), Vol. 285, Issue 5428, pp. 730-2, 1999 (PubMed).

Das, Sugita, Brenner: "Mechanisms of Vdelta1 gammadelta T cell activation by microbial components." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 11, pp. 6578-86, 2004 (PubMed).

Alternatives for antigen "Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1)", type "Antibodies"
Hosts (46), (45), (32), (27), (9), (1)
Reactivities (94), (81), (20)
Applications (134), (28), (27), (21), (13), (10), (9), (4), (4), (4), (3), (1)
Conjugates (20), (17), (13), (7), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (8), (4)
Beacon