Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1) antibody

Antigen: Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1)

Synonyms: KLR, CD314, NKG2D, NKG2-D, FLJ17759, FLJ75772, D12S2489E, KLRK1, Nkrp2, Nkg2d, D6H12S2489E

Clonality: Monoclonal (5C6)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunoprecipitation (IP), Flow Cytometry (FACS)

Catalog no.: ABIN120291

Additional Information

Alternative name: CD314 / KLRK1

UniProt: P26718

Immunogen: NKL cells. Spleen cells from immunised RBF/DnJ mice were fused with cells of the p3 mouse myeloma cell line

Cross-Reactivity: Human

Isotype: IgG2a

Clone: 5C6

Description: CD314 is a C-type lectin-like activating receptor which is expressed on most natural killer (NK) cells, CD8 T cells and gamma delta T cells. CD314 forms homodimers that signal through an associated DAP10 adaptor protein. Ligands of CD314 include MICA, MICB and UL16 binding protein (ULBP), which are inducibly expressed. Ligand binding to CD314 results in NK cell activation and potent costimulation of effector T cells. Alternate Names: D12S2489E, NKG2D, KLRK1, NKG2-D-activating NK Receptor, NK Cell Receptor D, Killer cell lectin- like receptor subfamily K member 1, NKG2-D type II integral membrane protein.

Application Details

Application Notes: Flow Cytometry: Use 10 ul of 1/100-1/200 diluted antibody to label 10e6 cells in 100 ul. Immunoprecipitation. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Purified

Buffer: PBS, pH 7.4 containing 0.09% Sodium Azide as preservative.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Restrictions: For Research Use only

Publications

Bauer, Groh, Wu et al.: "Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA." in: Science (New York, N.Y.), Vol. 285, Issue 5428, pp. 727-9, 1999 (PubMed).

Wu, Song, Bakker et al.: "An activating immunoreceptor complex formed by NKG2D and DAP10." in: Science (New York, N.Y.), Vol. 285, Issue 5428, pp. 730-2, 1999 (PubMed).

Das, Sugita, Brenner: "Mechanisms of Vdelta1 gammadelta T cell activation by microbial components." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 11, pp. 6578-86, 2004 (PubMed).