Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1) antibody

Details for Product No. ABIN120291
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Antigen
Synonyms KLRK1, NKG2D, CD314, D12S2489E, KLR, NKG2-D, D6H12S2489E, Nkg2d, Nkrp2
Reactivity
Human
(111), (71), (15)
Host
Mouse
(56), (39), (38), (29), (7), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(25), (22), (11), (8), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Flow Cytometry (FACS), Immunoprecipitation (IP)
(142), (30), (26), (25), (11), (11), (10), (9), (3), (2), (2), (1)
Pubmed 3 references available
Quantity 0.2 mg
Shipping to United States (Change)
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Catalog No. ABIN120291
511.50 $
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Immunogen NKL cells. Spleen cells from immunised RBF/DnJ mice were fused with cells of the p3 mouse myeloma cell line.
Clone 5C6
Isotype IgG2a
Specificity This antibody is specific for CD314, also known as natural killer receptor G2 (NKG2D) and as killer cell lectin-like receptor subfamily K, member 1 (KLRK1). Clone 5C6 is reported to inhibit T cell recognition of MICA (1, 3). We recommend the use of SM2259LE for such studies.
Purification Affinity Chromatography on Protein G.
Alternative Name CD314 / KLRK1
Background CD314 is a C-type lectin-like activating receptor which is expressed on most natural killer (NK) cells, CD8 T cells and gamma delta T cells. CD314 forms homodimers that signal through an associated DAP10 adaptor protein. Ligands of CD314 include MICA, MICB and UL16 binding protein (ULBP), which are inducibly expressed. Ligand binding to CD314 results in NK cell activation and potent costimulation of effector T cells.Synonyms: D12S2489E, Killer cell lectin-like receptor subfamily K member 1, NK cell receptor D, NKG2-D type II integral membrane protein, NKG2-D-activating NK receptor, NKG2D
Gene ID 100528032
NCBI Accession NP_001186734
UniProt P26718
Application Notes Flow Cytometry: Use 10 μL of 1/100-1/200 diluted antibody to label 10^6 cells in 100 μL. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Bauer, Groh, Wu et al.: "Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA." in: Science (New York, N.Y.), Vol. 285, Issue 5428, pp. 727-9, 1999 (PubMed).

Wu, Song, Bakker et al.: "An activating immunoreceptor complex formed by NKG2D and DAP10." in: Science (New York, N.Y.), Vol. 285, Issue 5428, pp. 730-2, 1999 (PubMed).

Das, Sugita, Brenner: "Mechanisms of Vdelta1 gammadelta T cell activation by microbial components." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 11, pp. 6578-86, 2004 (PubMed).

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