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Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1) antibody (FITC)

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Antigen
  • CD314
  • D6H12S2489E
  • D12S2489E
  • KLR
  • KLRK1
  • NKG2-D
  • Nkg2d
  • NKG2D
  • Nkrp2
Reactivity
Human
150
64
18
2
2
2
Host
Mouse
101
33
30
17
13
3
Clonality (Clone)
Monoclonal ()
Conjugate
FITC
26
20
13
7
5
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
139
54
44
41
34
32
24
11
10
3
3
3
3
2
2
2
1
1
Supplier
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Immunogen NKL cells. Spleen cells from immunised RBF/DnJ mice were fused with cells of the p3 mouse myeloma cell line.
Clone 1D11
Isotype IgG1
Specificity This antibody is specific for CD314 also known as natural killer receptor G2 (NKG2D) and as killer cell lectin-like receptor subfamily K, member 1 (KLRK1). Clone 1D11 is reported to inhibit T cell recognition of MICA (1,6). We recommend the use of SM2260LE for such studies.
Purification Affinity Chromatography on Protein G.
Alternative Name CD314 / KLRK1 (KLRK1 Antibody Abstract)
Background CD314 is a C-type lectin-like activating receptor which is expressed on most natural killer (NK) cells, CD8 T cells and gamma delta T cells. CD314 forms homodimers that signal through an associated DAP10 adaptor protein. Ligands of CD314 include MICA, MICB and UL16 binding protein (ULBP), which are inducibly expressed. Ligand binding to CD314 results in NK cell activation and potent costimulation of effector T cells.Synonyms: D12S2489E, Killer cell lectin-like receptor subfamily K member 1, NK cell receptor D, NKG2-D type II integral membrane protein, NKG2-D-activating NK receptor, NKG2D
Gene ID 100528032
NCBI Accession NP_001186734
UniProt P26718
Application Notes Flow Cytometry (Neat-1/10): Use 10 μL of the suggested working dilution to label 106 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.1 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Das, Sugita, Brenner: "Mechanisms of Vdelta1 gammadelta T cell activation by microbial components." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 11, pp. 6578-86, 2004 (PubMed).

Groh, Bruhl, El-Gabalawy, Nelson, Spies: "Stimulation of T cell autoreactivity by anomalous expression of NKG2D and its MIC ligands in rheumatoid arthritis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 16, pp. 9452-7, 2003 (PubMed).

Jinushi, Takehara, Tatsumi, Kanto, Groh, Spies, Suzuki, Miyagi, Hayashi: "Autocrine/paracrine IL-15 that is required for type I IFN-mediated dendritic cell expression of MHC class I-related chain A and B is impaired in hepatitis C virus infection." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 10, pp. 5423-9, 2003 (PubMed).

Groh, Rhinehart, Randolph-Habecker, Topp, Riddell, Spies: "Costimulation of CD8alphabeta T cells by NKG2D via engagement by MIC induced on virus-infected cells." in: Nature immunology, Vol. 2, Issue 3, pp. 255-60, 2001 (PubMed).

Bauer, Groh, Wu, Steinle, Phillips, Lanier, Spies: "Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA." in: Science (New York, N.Y.), Vol. 285, Issue 5428, pp. 727-9, 1999 (PubMed).

Wu, Song, Bakker, Bauer, Spies, Lanier, Phillips: "An activating immunoreceptor complex formed by NKG2D and DAP10." in: Science (New York, N.Y.), Vol. 285, Issue 5428, pp. 730-2, 1999 (PubMed).