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Notch 1 (NOTCH1) antibody (PE)

Antigen

Notch 1 (NOTCH1)
Synonyms hN1, TAN1, Mis6, Tan1, lin-12, 9930111A19Rik, NOTCH, NOTCH1, notch1, hn1, tan1, notch, xotch, xnotch, notch-1, xnotch1
Clonality Monoclonal (MN1A)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human, Mouse (Murine)
Conjugate
Application
Alternatives Flow Cytometry (FACS)
4 references available
Catalog no. ABIN120355
Quantity 100 tests  (0.1 mg/ml)  (Variants)
Price 269.50 $   Plus shipping costs $45.00
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anti-Notch 1 (NOTCH1) antibody (PE)

Additional Information
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Alternative name NOTCH1
UniProt P46531
Immunogen Synthetic peptide corresponding to cdc10-NCR region within mouse Notch1. Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Cross-Reactivity Human, Mouse (Murine)
Isotype IgG1
Clone MN1A
Description Notch1 is a single transmembrane receptor which is expressed in a range of cells including hematopoietic cells in mouse fetal liver, adult thymus and bone marrow. Notch1 plays a role in embryonic development and tissue homeostasis in the adult. Recent work has suggested a further role for this receptor in the regulation of lymphopoieisis and myelopoiesis. Following ligand binding, Notch1 is cleaved in the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus, where it plays a role in the modulation of gene expression. Alternate Names: Notch1, NOTCH-1, TAN1, Neurogenic Locus Notch Homolog Protein 1, Translocation-associated Notch Protein TAN-1.

Application Details
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Application Notes Flow Cytometry: Use 10 ul of Neat-1/10 diluted antibody to label 1x10e6 cells in 100 ul. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Concentration 0.1 mg/ml
Buffer PBS, pH 7.4, containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer.
Storage Prior to and following reconstitution store the antibody at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light. Shelf Life: one year from despatch.Reconstitution: Restore with 1 ml distilled water..
Research Area Receptors, Signaling, Inflammation, Immunology
Restrictions For Research Use only

Publications
Application Details Images Alternatives Variants back to top

Ray, Yao, Nowotny et al.: "Evidence for a physical interaction between presenilin and Notch." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 6, pp. 3263-8, 1999 (PubMed).

Huppert, Le, Schroeter et al.: "Embryonic lethality in mice homozygous for a processing-deficient allele of Notch1." in: Nature, Vol. 405, Issue 6789, pp. 966-70, 2000 (PubMed).

Espinosa, Santos, Inglés-Esteve et al.: "p65-NFkappaB synergizes with Notch to activate transcription by triggering cytoplasmic translocation of the nuclear receptor corepressor N-CoR." in: Journal of cell science, Vol. 115, Issue Pt 6, pp. 1295-303, 2002 (PubMed).

Kang-Decker, Tong, Boussouar et al.: "Loss of CBP causes T cell lymphomagenesis in synergy with p27Kip1 insufficiency." in: Cancer cell, Vol. 5, Issue 2, pp. 177-89, 2004 (PubMed).