Search, Find and Buy Antibodies, ELISA Kits and Proteins.
Loading Loading
Order hotline:
phone +1 404 474 4654
fax +1 888 205 9894 (TF)
Details for Product No. ABIN120355

Notch 1 (NOTCH1) antibody (PE)

Request
Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms hN1, TAN1, 9930111A19Rik, Mis6, N1, Tan1, lin-12, NOTCH, Xotch, notch, notch-1, notch1-a, xnotch, xnotch1, notch1, NOTCH1, hn1, tan1, xotch
Reactivity
»Alternatives Human, Mouse (Murine)
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
Application
»Alternatives Flow Cytometry (FACS)
Pubmed 5 references available
Catalog no. ABIN120355
Quantity 100 tests
Price
275.00 $   Plus shipping costs $45.00
Shipping to
Availability Will be delivered in 6 to 8 Business Days
Immunogen Synthetic peptide corresponding to cdc10-NCR region within mouse Notch1. Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Clone MN1A
Isotype IgG1
Specificity This antibody is specific for Notch1.
Purification Affinity Chromatography on Protein G
Alternative Name NOTCH1
Background Notch1 is a single transmembrane receptor which is expressed in a range of cells including hematopoietic cells in mouse fetal liver, adult thymus and bone marrow. Notch1 plays a role in embryonic development and tissue homeostasis in the adult. Recent work has suggested a further role for this receptor in the regulation of lymphopoieisis and myelopoiesis. Following ligand binding, Notch1 is cleaved in the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus, where it plays a role in the modulation of gene expression.
Alternate names: Neurogenic locus notch homolog protein 1, Notch 1, TAN1, hN1
Gene ID 4851
NCBI Accession NP_060087.3
UniProt P46531
Research Area Receptors, Signaling, Inflammation, Immunology
Application Notes Flow Cytometry: Use 10 ul of Neat-1/10 diluted antibody to label 1x10e6 cells in 100 ul. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore with 1 mL distilled water.
Concentration 0.1 mg/mL
Buffer PBS, pH 7.4, containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer.
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Original vial: 1 year at 2-8°C. After reconstitution, aliquot and freeze stock solution at -20°C.
Expiry Date 12 months
Background publications Ray, Yao, Nowotny et al.: "Evidence for a physical interaction between presenilin and Notch." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 6, pp. 3263-8, 1999 (PubMed).

Huppert, Le, Schroeter et al.: "Embryonic lethality in mice homozygous for a processing-deficient allele of Notch1." in: Nature, Vol. 405, Issue 6789, pp. 966-70, 2000 (PubMed).

Espinosa, Santos, Inglés-Esteve et al.: "p65-NFkappaB synergizes with Notch to activate transcription by triggering cytoplasmic translocation of the nuclear receptor corepressor N-CoR." in: Journal of cell science, Vol. 115, Issue Pt 6, pp. 1295-303, 2002 (PubMed).

Kang-Decker, Tong, Boussouar et al.: "Loss of CBP causes T cell lymphomagenesis in synergy with p27Kip1 insufficiency." in: Cancer cell, Vol. 5, Issue 2, pp. 177-89, 2004 (PubMed).

Dass, Choong: "Zoledronic acid inhibits osteosarcoma growth in an orthotopic model." in: Molecular cancer therapeutics, Vol. 6, Issue 12 Pt 1, pp. 3263-70, 2007 (PubMed).

Alternatives for antigen "Notch 1 (NOTCH1)", type "Antibodies"
Hosts (85), (61), (5), (5), (3), (2)
Reactivities (119), (75), (29), (4), (2), (1), (1), (1), (1), (1)
Applications (91), (52), (41), (40), (27), (12), (10), (10), (5), (4), (2), (2), (2)
Conjugates (8), (7), (2), (1), (1), (1), (1), (1)
Epitopes (12), (9), (6), (5), (5), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Beacon