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CD45 antibody (Protein tyrosine Phosphatase, Receptor Type, C) (Biotin)

Details for Product anti-PTPRC Antibody No. ABIN120461, Supplier: Login to see
Antigen
  • PTPRC
  • si:dkeyp-47c5.1
  • B220
  • CD45
  • CD45R
  • GP180
  • L-CA
  • LCA
  • LY5
  • T200
  • Cd45
  • Ly-5
  • Lyt-4
  • loc
  • Lca
  • RT7
  • cd45
Reactivity
Rat (Rattus)
964
623
193
69
49
41
38
27
21
11
8
5
5
4
3
3
2
1
1
1
1
1
Host
Mouse
999
656
205
3
2
Clonality (Clone)
Monoclonal ()
Conjugate
This CD45 antibody is conjugated to Biotin
277
210
156
103
38
27
24
20
20
19
16
16
14
14
14
14
10
8
7
6
6
6
5
5
5
5
5
5
5
5
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
1477
328
322
281
227
206
188
110
78
47
40
29
26
12
11
10
7
7
6
6
4
4
2
2
2
2
2
2
2
2
1
1
1
1
1
Supplier
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Immunogen Rat thymocyte membrane glycoproteins. Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
Clone OX-1
Isotype IgG1
Specificity This antibody recognises CD45. Antibodies recognising a common epitope on all of these isoforms are termed CD45, whilst those recognising only individual isoforms are termed CD45RA, CD45RO etc. OX-1 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes.
Alternative Name CD45 / LCA (PTPRC Antibody Abstract)
Background The leucocyte common antigen consists of a family of heavily glycosylated membrane glycoproteins of molecular weight 180 - 240 kDa. CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction.Synonyms: L-CA, Leukocyte common antigen, PTPRC, Receptor-type tyrosine-protein phosphatase C, T200
Gene ID 24699
NCBI Accession NP_001103357
UniProt P04157
Pathways
Application Notes Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore with 1 mL distilled water. The addition of 0.1 % sodium azide is recommended after reconstitution.
Concentration 0.08 mg/mL
Buffer PBS, pH 7.4 containing 1 % Bovine Serum Albumin
Handling Advice This product is photosensitive and should be protected from light
Storage 4 °C
Storage Comment Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
Background publications Sato, Balla, Otterbein et al.: "Carbon monoxide generated by heme oxygenase-1 suppresses the rejection of mouse-to-rat cardiac transplants." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 6, pp. 4185-94, 2001 (PubMed).

Murakami, Sato, Nagasawa et al.: "Regulation of mast cell signaling through high-affinity IgE receptor by CD45 protein tyrosine phosphatase." in: International immunology, Vol. 12, Issue 2, pp. 169-76, 2000 (PubMed).

Martín, Molina, Bricio et al.: "Passive dual immunization against tumour necrosis factor-alpha (TNF-alpha) and IL-1 beta maximally ameliorates acute aminonucleoside nephrosis." in: Clinical and experimental immunology, Vol. 99, Issue 2, pp. 283-8, 1995 (PubMed).

Barclay: "The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues." in: Immunology, Vol. 42, Issue 4, pp. 593-600, 1981 (PubMed).

Woollett, Barclay, Puklavec et al.: "Molecular and antigenic heterogeneity of the rat leukocyte-common antigen from thymocytes and T and B lymphocytes." in: European journal of immunology, Vol. 15, Issue 2, pp. 168-73, 1985 (PubMed).

Barclay, Jackson, Willis et al.: "Lymphocyte specific heterogeneity in the rat leucocyte common antigen (T200) is due to differences in polypeptide sequences near the NH2-terminus." in: The EMBO journal, Vol. 6, Issue 5, pp. 1259-64, 1987 (PubMed).

Sunderland, McMaster, Williams: "Purification with monoclonal antibody of a predominant leukocyte-common antigen and glycoprotein from rat thymocytes." in: European journal of immunology, Vol. 9, Issue 2, pp. 155-9, 1979 (PubMed).