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CD45 antibody (Protein tyrosine Phosphatase, Receptor Type, C)

Details for Product anti-PTPRC Antibody No. ABIN120463, Supplier: Log in to see
Antigen
  • PTPRC
  • si:dkeyp-47c5.1
  • B220
  • CD45
  • CD45R
  • GP180
  • L-CA
  • LCA
  • LY5
  • T200
  • Cd45
  • Ly-5
  • Lyt-4
  • loc
  • Lca
  • RT7
  • cd45
Reactivity
Rat (Rattus)
940
614
184
69
49
41
38
32
21
11
8
5
5
4
4
3
3
2
1
1
1
1
1
Host
Mouse
974
642
201
2
2
Clonality (Clone)
Monoclonal ()
Conjugate
This CD45 antibody is un-conjugated
269
203
150
103
38
26
24
20
20
19
16
16
14
14
14
14
10
8
7
6
6
6
5
5
5
5
5
5
5
5
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
1442
326
324
281
210
201
177
110
83
47
40
35
26
12
11
10
7
7
6
6
4
4
2
2
2
2
2
2
2
2
1
1
1
1
1
Supplier
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Immunogen Rat thymocyte membrane glycoproteins
Clone OX-1
Isotype IgG1
Specificity SM274P recognises CD45, also known as the leucocyte common antigen (LCA). The leucocyte common antigen consists of a family of heavily glycosylated membrane glycoproteins of molecular weight 180 - 240 kDa. Antibodies recognizing a common epitope on all of these isoforms are termed CD45, whilst those recognising only individual isoforms are termed CD45RA, CD45RO etc. OX-1 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes. This product is routinely tested by flow cytometry on rat splenocytes.
Purification Protein G chromatography
Alternative Name CD45 / LCA (PTPRC Antibody Abstract)
Background CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction.Synonyms: L-CA, Leukocyte common antigen, PTPRC, Receptor-type tyrosine-protein phosphatase C, T200
Gene ID 24699
NCBI Accession NP_001103357
UniProt P04157
Application Notes Flow cytometry: use 10 μL of 1/100 diluted antibody to label 10^6 cells. Immunohistochemistry on frozen sections (3).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use
Storage 4 °C/-20 °C
Storage Comment Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
 image for anti-CD45 antibody (Protein tyrosine Phosphatase, Receptor Type, C) (ABIN120463) Staining of rat peripheral blood lymphocytes with MOUSE ANTI RAT CD45 (ABIN120463).
Background publications Sato, Balla, Otterbein et al.: "Carbon monoxide generated by heme oxygenase-1 suppresses the rejection of mouse-to-rat cardiac transplants." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 6, pp. 4185-94, 2001 (PubMed).

Martín, Molina, Bricio et al.: "Passive dual immunization against tumour necrosis factor-alpha (TNF-alpha) and IL-1 beta maximally ameliorates acute aminonucleoside nephrosis." in: Clinical and experimental immunology, Vol. 99, Issue 2, pp. 283-8, 1995 (PubMed).

Woollett, Barclay, Puklavec et al.: "Molecular and antigenic heterogeneity of the rat leukocyte-common antigen from thymocytes and T and B lymphocytes." in: European journal of immunology, Vol. 15, Issue 2, pp. 168-73, 1985 (PubMed).

Sunderland, McMaster, Williams: "Purification with monoclonal antibody of a predominant leukocyte-common antigen and glycoprotein from rat thymocytes." in: European journal of immunology, Vol. 9, Issue 2, pp. 155-9, 1979 (PubMed).

Standring, McMaster, Sunderland et al.: "The predominant heavily glycosylated glycoproteins at the surface of rat lymphoid cells are differentiation antigens." in: European journal of immunology, Vol. 8, Issue 12, pp. 832-9, 1979 (PubMed).