CD45 antibody (Protein tyrosine Phosphatase, Receptor Type, C)

Details for Product anti-PTPRC Antibody No. ABIN120463, Supplier: Log in to see
Antigen
  • PTPRC
  • si:dkeyp-47c5.1
  • B220
  • CD45
  • CD45R
  • GP180
  • L-CA
  • LCA
  • LY5
  • T200
  • Cd45
  • Ly-5
  • Lyt-4
  • loc
  • Lca
  • RT7
  • cd45
  • protein tyrosine phosphatase, receptor type, C
  • PTPRC
  • ptprc
  • Ptprc
Alternatives
anti-Human CD45 antibody for Immunocytochemistry
Reactivity
Rat (Rattus)
1449
761
189
128
68
48
45
43
23
20
19
16
6
6
4
4
4
3
3
3
2
2
1
1
1
1
1
1
Host
Mouse
1477
782
240
3
2
Clonality (Clone)
Monoclonal ()
Conjugate
This CD45 antibody is un-conjugated
266
234
178
100
70
63
63
55
48
42
39
35
34
34
34
29
29
29
29
22
21
17
15
14
14
13
13
13
12
10
9
8
7
6
6
6
5
5
5
5
5
5
5
4
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
2058
602
557
556
520
479
453
387
203
54
49
47
28
16
11
10
7
7
6
4
3
3
2
2
2
2
2
2
2
1
1
1
Options
Supplier
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Immunogen Rat thymocyte membrane glycoproteins
Clone OX-1
Isotype IgG1
Specificity SM274P recognises CD45, also known as the leucocyte common antigen (LCA). The leucocyte common antigen consists of a family of heavily glycosylated membrane glycoproteins of molecular weight 180 - 240 kDa. Antibodies recognizing a common epitope on all of these isoforms are termed CD45, whilst those recognising only individual isoforms are termed CD45RA, CD45RO etc. OX-1 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes. This product is routinely tested by flow cytometry on rat splenocytes.
Purification Protein G chromatography
Alternative Name CD45 / LCA (PTPRC Antibody Abstract)
Background CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction.Synonyms: L-CA, Leukocyte common antigen, PTPRC, Receptor-type tyrosine-protein phosphatase C, T200
Gene ID 24699
NCBI Accession NP_001103357
UniProt P04157
Pathways TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events
Application Notes Flow cytometry: use 10 μL of 1/100 diluted antibody to label 10^6 cells. Immunohistochemistry on frozen sections (3).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use
Storage 4 °C/-20 °C
Storage Comment Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
 image for anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) antibody (ABIN120463) Staining of rat peripheral blood lymphocytes with MOUSE ANTI RAT CD45 (ABIN120463).
Background publications Sato, Balla, Otterbein, Smith, Brouard, Lin, Csizmadia, Sevigny, Robson, Vercellotti, Choi, Bach, Soares: "Carbon monoxide generated by heme oxygenase-1 suppresses the rejection of mouse-to-rat cardiac transplants." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 6, pp. 4185-94, 2001 (PubMed).

Martín, Molina, Bricio, Mampaso: "Passive dual immunization against tumour necrosis factor-alpha (TNF-alpha) and IL-1 beta maximally ameliorates acute aminonucleoside nephrosis." in: Clinical and experimental immunology, Vol. 99, Issue 2, pp. 283-8, 1995 (PubMed).

Woollett, Barclay, Puklavec, Williams: "Molecular and antigenic heterogeneity of the rat leukocyte-common antigen from thymocytes and T and B lymphocytes." in: European journal of immunology, Vol. 15, Issue 2, pp. 168-73, 1985 (PubMed).

Standring, McMaster, Sunderland, Williams: "The predominant heavily glycosylated glycoproteins at the surface of rat lymphoid cells are differentiation antigens." in: European journal of immunology, Vol. 8, Issue 12, pp. 832-9, 1979 (PubMed).

Sunderland, McMaster, Williams: "Purification with monoclonal antibody of a predominant leukocyte-common antigen and glycoprotein from rat thymocytes." in: European journal of immunology, Vol. 9, Issue 2, pp. 155-9, 1979 (PubMed).

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