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CD45 antibody (Protein tyrosine Phosphatase, Receptor Type, C) (PE)

Details for Product anti-PTPRC Antibody No. ABIN120466, Supplier: Log in to see
Antigen
  • B220
  • CD45
  • Cd45
  • cd45
  • CD45R
  • GP180
  • L-CA
  • Lca
  • LCA
  • loc
  • Ly-5
  • LY5
  • Lyt-4
  • PTPRC
  • RT7
  • si:dkeyp-47c5.1
  • T200
Reactivity
Rat (Rattus)
1213
716
215
143
62
51
41
39
24
21
18
8
5
5
4
3
3
2
1
1
1
1
1
Host
Mouse
1289
789
203
2
2
Clonality (Clone)
Monoclonal ()
Conjugate
This CD45 antibody is conjugated to PE
281
226
168
103
48
48
47
41
40
29
27
27
26
26
25
24
24
24
24
20
17
17
16
16
14
14
10
9
9
7
6
6
6
5
5
5
5
5
5
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
1822
510
495
459
429
375
322
217
189
48
46
40
26
12
11
10
7
7
6
6
4
4
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Supplier
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Immunogen Rat thymocyte membrane glycoproteins.
Clone OX-1
Isotype IgG1
Specificity This antibody recognises CD45. OX-1 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes.
Alternative Name CD45 / LCA (PTPRC Antibody Abstract)
Background CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognising a common epitope on all of the isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.Synonyms: L-CA, Leukocyte common antigen, PTPRC, Receptor-type tyrosine-protein phosphatase C, T200
Gene ID 24699
NCBI Accession NP_001103357
UniProt P04157
Application Notes Flow cytometry: use 10 μL of neat antibody to label 10^6 cells in 100 μL. (This product is routinely tested in flow cytometry on rat splenocytes).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore with 1 mL distilled water.
Buffer PBS, pH 7.4, containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice This product is photosensitive and should be protected from light
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
Supplier Images
 image for anti-CD45 antibody (Protein tyrosine Phosphatase, Receptor Type, C)  (PE) (ABIN120466) Staining of rat spleen cells with MOUSE ANTI RAT CD45: RPE (ABIN120466).
Product cited in: Eipel, Bordel, Nickels, Menger, Vollmar: "Impact of leukocytes and platelets in mediating hepatocyte apoptosis in a rat model of systemic endotoxemia." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 286, Issue 5, pp. G769-76, 2004

Background publications Sato, Balla, Otterbein, Smith, Brouard, Lin, Csizmadia, Sevigny, Robson, Vercellotti, Choi, Bach, Soares: "Carbon monoxide generated by heme oxygenase-1 suppresses the rejection of mouse-to-rat cardiac transplants." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 6, pp. 4185-94, 2001

Murakami, Sato, Nagasawa, Yamashita: "Regulation of mast cell signaling through high-affinity IgE receptor by CD45 protein tyrosine phosphatase." in: International immunology, Vol. 12, Issue 2, pp. 169-76, 2000

Martín, Molina, Bricio, Mampaso: "Passive dual immunization against tumour necrosis factor-alpha (TNF-alpha) and IL-1 beta maximally ameliorates acute aminonucleoside nephrosis." in: Clinical and experimental immunology, Vol. 99, Issue 2, pp. 283-8, 1995

Woollett, Barclay, Puklavec, Williams: "Molecular and antigenic heterogeneity of the rat leukocyte-common antigen from thymocytes and T and B lymphocytes." in: European journal of immunology, Vol. 15, Issue 2, pp. 168-73, 1985

Sunderland, McMaster, Williams: "Purification with monoclonal antibody of a predominant leukocyte-common antigen and glycoprotein from rat thymocytes." in: European journal of immunology, Vol. 9, Issue 2, pp. 155-9, 1979