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Antibodies
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anti-T Cell Receptor (TCR) Vb 16 antibody
T Cell Receptor (TCR) Vb 16 antibody
| Antigen |
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| Clonality |
Monoclonal (HIS42) |
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Host
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Mouse
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Reactivity
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Rat (Rattus)
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Application
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Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP)
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| Catalog no. |
ABIN120476 |
| Quantity |
2 ml |
| Price |
250.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Immunogen
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PVG RT7.2 Rat Thymocytes. Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line
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Format
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Supernatant
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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HIS42
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Description
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The subpopulation of HIS 42 positive T cells contains both CD4 and CD8 positive cells, in the same ratio as found in the peripheral T cell population. When bound to Sepharose beads, HIS 42 induces T cell proliferation in the presence of IL-2. In contrast to lymph node T cells a number of thymocytes are found to express HIS 42 only in the cytoplasm or together with membrane expression. Most bright HIS 42 surface labelled thymocytes are also positive for MRC OX-44, a marker predominantly identifying mature thymocytes. Alternate Names: TCR V Beta 16 (16.1).
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Specificity
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This antibody detects a minor subset of T cells. SDS PAGE analysis of the membrane molecules immunoprecipitated by HIS 42 shows a band at 85kD. On reduction it separates to two bands at 35kD and 48kD. From this data it is concluded that HIS 42 is directed against a determinant on the rat T cell receptor, which is common to a small number of T cells. Preliminary data suggests that the determinant is found on the beta chain, associated with the Vbeta16.1 gene product.
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Application Notes
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Flow Cytometry. Immunoprecipitation. Immunohistochemistry on frozen sections: 1/10. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Buffer
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This antibody is supplied as liquid, Tissue Culture Supernatant with 0.09% sodium azide as preservative.
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Storage
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Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
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Research Area
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Immunology, Receptors
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Restrictions
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For Research Use only
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