CD63 antibody
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- Target See all CD63 Antibodies
- CD63
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD63 antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
- Specificity
- The antibody reacts with CD63 (LAMP-3), a 40-60 kDa tetraspan glycoprotein expressed by granulocytes, platelets, T cells, monocytes/macrophages and endothelial cells. Cell surface exposition of CD63 is usually activation-dependent.
- Cross-Reactivity (Details)
- Species reactivity (tested):Human.
- Purification
- Affinity Chromatography on Protein A
- Immunogen
- HPB-ALL T cell line
- Clone
- MEM-259
- Isotype
- IgG1
- Top Product
- Discover our top product CD63 Primary Antibody
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- Application Notes
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Flow cytometry (detection of activated platelets, neutrophils and basophils). Immunoprecipitation. Western blotting under non-reducing conditions. Immunocytochemistry. Immunohistochemistry (paraffin sections): Recommended dilution:10 m g/mL, Positive tissue: spleen
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH ~7.4, 15 mM Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C/-20 °C
- Storage Comment
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Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch. General. - Expiry Date
- 12 months
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Effector granules in human T lymphocytes: the luminal proteome of secretory lysosomes from human T cells." in: Cell communication and signaling : CCS, Vol. 9, Issue 1, pp. 4, (2011) (PubMed).
: "
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Effector granules in human T lymphocytes: the luminal proteome of secretory lysosomes from human T cells." in: Cell communication and signaling : CCS, Vol. 9, Issue 1, pp. 4, (2011) (PubMed).
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- Target
- CD63
- Alternative Name
- CD63 (CD63 Products)
- Synonyms
- C75951 antibody, ME491 antibody, Tspan30 antibody, LAMP-3 antibody, MLA1 antibody, OMA81H antibody, TSPAN30 antibody, TSP29FA antibody, CD63 antigen antibody, CD63 molecule antibody, Cd63 molecule antibody, Cd63 antibody, CD63 antibody
- Background
- CD63, a glycoprotein of tetraspanin family, is present in late endosomes, lysosomes and secretory vesicles of various cell types. It is also present in the plasma membrane, usually following cell activation. Hence, it has become an widely used basophil activation marker. In mast cells, however, CD63 exposition does not need their activation. CD63 interacts with integrins and affects phagocytosis and cell migration, it is also involved in H/K-ATPase trafficking regulation of ROMK1 channels. CD63 also serves as a T-cell costimulation molecule. Expression of CD63 can be used for predicting the prognosis in earlier stages of carcinomas.Synonyms: Granulophysin, ME491, MLA1, Melanoma-associated antigen ME491, OMA81H, Ocular melanoma-associated antigen, TSPAN30, Tetraspanin-30
- Gene ID
- 967
- UniProt
- P08962
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