Immunoprecipitation. Immunocytochemistry. Western Blot: 2-3 mg/mL, 60 min in RT, Boil for 3 min, Resuspend dry pelet in solubilizelysis buffer (with carbamide and detergent NP-40), Mix lysate with reducing SDS-PAGE (+merkaptoethanol) sample buffer, Reducing conditions. Positive control: HeLa human cell line. Immunohistochemistry on paraffin sections: 20 mg/mL, 0,1 % pepsin, 30 min, 37C, 4Covernight, chromogen DAB. Positive tissue: pancreas. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS, pH ~7.4, 15 mM Sodium Azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues. Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.Synonyms: CK-7, CK7, K-7, K7, KRT-7, KRT7, Keratin type II cytoskeletal 7, Keratin-7, SCL, Sarcolectin