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Mouse anti-Human IgG Antibody

WB, FACS, IF, EIA Monoclonal 4A11 IgG2b unconjugated
Catalog No. ABIN120644
  • Target See all IgG products
    IgG
    Reactivity
    • 692
    • 672
    • 559
    • 452
    • 366
    • 239
    • 198
    • 147
    • 137
    • 131
    • 122
    • 98
    • 95
    • 95
    • 92
    • 62
    • 46
    • 33
    • 27
    • 17
    • 12
    • 10
    • 8
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 1718
    • 1474
    • 537
    • 203
    • 118
    • 103
    • 33
    • 31
    • 21
    • 17
    • 15
    • 11
    • 11
    • 1
    Mouse
    Clonality
    • 3347
    • 164
    • 1
    Monoclonal
    Application
    • 1837
    • 1789
    • 1787
    • 1499
    • 962
    • 934
    • 913
    • 515
    • 234
    • 115
    • 115
    • 111
    • 100
    • 77
    • 59
    • 50
    • 50
    • 49
    • 32
    • 27
    • 25
    • 21
    • 17
    • 16
    • 11
    • 8
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Enzyme Immunoassay (EIA)
    Purification
    Precipitation Methods and Ion Exchange Chromatography.
    Immunogen
    Purified human IgG
    Clone
    4A11
    Isotype
    IgG2b
  • Application Notes
    ELISA. Western blotting. Has been described for previous lots to work in Flow Cytometry and Immunofluorescence.

    Other applications not tested.
    Otimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH ~7.4 with 15 mM Sodium Azide as preservative.
    Preservative
    Sodium azide
    Precaution of Use
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody at 2-8 °C for one month or (in aliquots) at -28 °C for longer.
  • Target
    IgG
    Abstract
    IgG Products
    Target Type
    Antibody
    Background
    Immunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effector molecule of the humoral immune response in man. Its concentration in blood plasma of healthy individuals is approximately 10 g/l, which accounts for about 75 % of the total plasma immunoglobulins. IgG has the highest stability of blood immunoglobulins (T1/2 = 21 days) and is able of placental transfer. IgG is secreted by plasma cells at a comparably high rate as other immunoglobulins.Synonyms: Human Immunoglobulin G
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