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PAG1 antibody (phosphoprotein Associated with Glycosphingolipid Microdomains 1) (AA 97-432)

Details for Product anti-PAG1 Antibody No. ABIN120652, Supplier: Log in to see
Antigen
  • MGC80192
  • CBP
  • PAG
  • Cbp
  • F730007C19Rik
  • Pag
  • 8430414N03Rik
  • IGFBP-4ase
  • PAG1
  • PAPP-A
Alternatives
anti-Human PAG1 antibody for Immunohistochemistry (Paraffin-embedded Sections)
Epitope
AA 97-432
20
16
3
2
1
1
1
Reactivity
Human
57
30
22
5
Host
Mouse
37
24
Clonality (Clone)
Monoclonal ()
Conjugate
This PAG1 antibody is un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
38
28
22
11
7
7
3
1
Supplier
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Immunogen Recombinant intracellular fragment (aa 97-432) of human Cbp/PAG
Clone MEM-255
Isotype IgG2a
Specificity The antibody recognizes an epitope (aa 235-280) of Csk-binding protein (Cbp) located in the cytoplasmic domain, also known as protein associated with glycosphingolipid-enriched microdomains (PAG).
Purification Protein-A affinity chromatography
Purity > 95 % pure (by SDS-PAGE)
Alternative Name PAG1 / Csk-Binding Protein (PAG1 Antibody Abstract)
Background PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.Synonyms: CBP, PAG, Phosphoprotein associated with glycosphingolipid-enriched microdomains 1, Transmembrane adapter protein PAG, Transmembrane phosphoprotein Cbp
Gene ID 55824
UniProt Q9NWQ8
Pathways p53 Signaling, EGFR Signaling Pathway
Application Notes Flow Cytometry: 2 mg/mL (intracellular staining, permeabilization required). Western Blotting: Csk binding protein is an ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane microdomains (rafts), which however migrates on SDS- PAGE gels anomalously as an 80 kDa molecule. Immunohistochemistry (paraffin sections).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.
Expiry Date 12 months
Background publications Brdicková, Brdicka, Andera et al.: "Interaction between two adapter proteins, PAG and EBP50: a possible link between membrane rafts and actin cytoskeleton." in: FEBS letters, Vol. 507, Issue 2, pp. 133-6, 2001 (PubMed).