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SIT1 antibody

SIT1 Reactivity: Human WB, IP, FACS Host: Mouse Monoclonal SIT-01 unconjugated
Catalog No. ABIN120658
  • Target See all SIT1 Antibodies
    SIT1 (Signaling threshold Regulating Transmembrane Adaptor 1 (SIT1))
    Reactivity
    • 22
    • 2
    Human
    Host
    • 17
    • 6
    • 1
    Mouse
    Clonality
    • 18
    • 6
    Monoclonal
    Conjugate
    • 11
    • 5
    • 2
    • 2
    • 2
    • 2
    This SIT1 antibody is un-conjugated
    Application
    • 21
    • 14
    • 6
    • 4
    • 3
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS)
    Specificity
    The antibody reacts with SHP2-interacting transmembrane adaptor protein (SIT) expressed exclusively in lymphoid organs.
    Characteristics
    Synonyms: Signaling threshold-regulating transmembrane adapter 1, SHP2-interacting transmembraneadapter protein, Suppression-inducing transmembrane adapter 1
    Purification
    Protein-A affinity chromatography
    Purity
    > 95 % pure (by SDS-PAGE)
    Immunogen
    Bacterially produced recombinant intracellular fragment of human SIT.
    Clone
    SIT-01
    Isotype
    IgG1
    Top Product
    Discover our top product SIT1 Primary Antibody
  • Application Notes
    Flow Cytometry (intracellular staining). Immunoprecipitation. Western Blotting: Western blot analysis demonstrated that SIT is expressed as anapproximately 40 kDa protein that is reduced to approximately 20 kDa by endoglycosidasetreatment.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
    Shelf life: one year from despatch.
    Expiry Date
    12 months
  • Target
    SIT1 (Signaling threshold Regulating Transmembrane Adaptor 1 (SIT1))
    Alternative Name
    SIT1 / SIT (SIT1 Products)
    Synonyms
    SIT1 antibody, RP11-331F9.5 antibody, SIT antibody, SIT-R antibody, Sit antibody, signaling threshold regulating transmembrane adaptor 1 antibody, suppression inducing transmembrane adaptor 1 antibody, SIT1 antibody, Sit1 antibody
    Background
    SIT (SHP2-interacting transmembrane adaptor protein) is expressed exclusively in lymphoid organs and acts either as a positive or as a negative regulatory element in T cell activation and in T cell development. Binding to Grb2 plays a pivotal role in signal transduction. Hubener et al. (2001) determined that the SIT gene contains 5 exons and spans 1.8 kb of genomic DNA. The SIT promoter demonstrated strong transcriptional activity and potential binding sites for both ubiquitous and lymphoid-specific transcription factors.Synonyms: SHP2-interacting transmembrane adapter protein, Signaling threshold-regulating transmembrane adapter 1, Suppression-inducing transmembrane adapter 1
    Gene ID
    27240
    UniProt
    Q9Y3P8
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