The antibody reacts with intra-acrosomal proteins (92 and 42 kDa) of sperms.
Characteristics
Synonyms: Acrosomal protein SP-10, SP10, SPACA2, Acrosomal vesicle protein 1, Intra-acrosomalSperm Protein
Purification
Protein-A affinity chromatography
Purity
> 95 % pure (by SDS-PAGE)
Immunogen
Freshly ejaculated dog sperms were washed in PBS and extracted in 3% acetic acid, 10%glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0. 2% acetic acid,lyophylized and subsequently used for immunization.
Immunocytochemistry, membrane permeabilization (acetone) essential. Western Blotting. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1 mg/mL
Buffer
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing. Shelf life: one year from despatch.
Canine intra-acrosomal sperm protein (92 and 42 kDa) is a testis-specific acrosomal vesicle protein 1 associated with the acrosomal membranes and matrix of the mature sperm.Synonyms: Acrosomal protein SP-10, Acrosomal vesicle protein 1, Intra-acrosomal Sperm Protein, SP10, SPACA2