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Antibodies
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anti-Selectin P Ligand (SELPLG) antibody
Selectin P Ligand (SELPLG) antibody
| Antigen |
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| Synonyms |
CLA, CD162, PSGL1, PSGL-1, SELPLG, SELPG |
| Clonality |
Monoclonal (TB5) |
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Host
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Reactivity
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Application
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 Alternatives Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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| Catalog no. |
ABIN120689 |
| Quantity |
0.1 mg (1.0 mg/ml) (Variants) |
| Price |
265.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Alternative name
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CD162 / PSGL1
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Swiss-Prot
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Q14242
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Isotype
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IgG1
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Clone
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TB5
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Description
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CD162 (P-selectin glycoprotein ligand-1, PSGL-1) is a sialomucin constitutively expressed as a disulfide-linked homodimer of two 120 kDa subunits on the surface of circulating leukocytes. CD162 serves as a ligand for P- E- and L-selectin, with the highest affinity for P-selectin. It is thus involved in leukocyte rolling at the endothelial surfaces, prerequisite for firm leukocyte adhesion and subsequent transendothelial migration. CD162 also mediates leukocyte-platelet adhesion and interleukocyte contacts. Whereas serving as an adhession molecule on mature leukocytes, CD162 is a potent negative regulator of human hematopoietic progenitors. Alternate Names: PSGL-1.
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Specificity
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The antibody TB5 reacts with CD162, a 220 kDa type I integral membrane protein expressed as disulfide-linked homodimer (sialomucin family). CD162 is present on the most peripheral blood T lymphocytes, monocytes, granulocytes, it is also expressed on a subpopulation of B lymphocytes and CD34+ bone marrow cells.
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Application Notes
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Suitable for Flow Cytometry analysis of human blood cells using 20 m l reagent / 100 m l of whole blood or 10e6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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1.0 mg/ml
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Buffer
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PBS, pH 7.4 with 15 mM sodium azide as preservative and 0.2% (w/v) high- grade BSA (Protease free) as stabilizer.
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Storage
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Store the antibody in the dark at 2-8°C. Do Not Freeze! Avoid prolonged exposure to light. Shelf Life: One year from despatch.
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Research Area
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CD Antigens, Surface Receptors of Immune Cells
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Restrictions
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For Research Use only
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Alternatives for antigen "Selectin P Ligand (SELPLG)", type "Antibodies"
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Hosts
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Mouse (28), Rat (1)
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Reactivities
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Human (28), Mouse (Murine) (1)
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Applications
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Flow Cytometry (FACS) (25), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (10), Western Blotting (WB) (8), Immunohistochemistry (Frozen Sections) (IHC (fro)) (7), Functional Studies (Func) (3), Blocking Antibody (Inhibition) (2), ELISA (1)
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Conjugates
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FITC (4), APC (3), PE (2), Alexa Fluor 700 (1)
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