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LAT2 antibody (AA 196-212)

LAT2 Reactivity: Human WB, IP, IHC (p) Host: Mouse Monoclonal NAP-03 unconjugated
Catalog No. ABIN120728
  • Target See all LAT2 Antibodies
    LAT2 (Linker For Activation of T Cells Family, Member 2 (LAT2))
    Binding Specificity
    • 8
    • 8
    • 7
    • 7
    • 7
    • 7
    • 6
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 196-212
    Reactivity
    • 54
    • 24
    • 15
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 48
    • 14
    • 1
    Mouse
    Clonality
    • 49
    • 14
    Monoclonal
    Conjugate
    • 40
    • 6
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This LAT2 antibody is un-conjugated
    Application
    • 52
    • 22
    • 17
    • 13
    • 9
    • 7
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Specificity
    The antibody recognizes the defined epitope (aa 196-212) of Non-T cell activation linker (NTAL), also known as LAB (linker of activated B cells), a 30 kDa transmembrane adaptor protein present in membrane microdomains (rafts) of B cells, NK cells and myeloid cells.
    Purification
    Protein A affinity chromatography
    Purity
    > 95 % (by SDS-PAGE)
    Immunogen
    Peptide corresponding to aa 196-212 of human NTAL coupled to KLH
    Clone
    NAP-03
    Isotype
    IgG1
    Top Product
    Discover our top product LAT2 Primary Antibody
  • Application Notes
    Immunoprecipitation. Western Blot: Recommended dilution: 1-2 mg/mL, incubation 60 min. Positive control: RAMOS human lymphoma cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non- reducing SDS-PAGE sample buffer. Application note: Non-Reducing conditions. 12 % separating SDS-PAGE gel.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
    Shelf Life: one year from despatch.
    Expiry Date
    12 months
  • Target
    LAT2 (Linker For Activation of T Cells Family, Member 2 (LAT2))
    Alternative Name
    LAT2 / WBSCR15 (LAT2 Products)
    Synonyms
    LAB antibody, NTAL antibody, WBSCR15 antibody, WBSCR5 antibody, WSCR5 antibody, LAT2 antibody, MGC139435 antibody, Ntal antibody, Wbscr5 antibody, AW125574 antibody, Wbscr15 antibody, linker for activation of T-cells family member 2 antibody, linker for activation of T cells family, member 2 antibody, LAT2 antibody, Lat2 antibody
    Background
    NTAL (Non-T cell Activation Linker), also known as LAB (Linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function.Synonyms: HSPC046, LAB, LLinker for activation of B-cells, Linker for activation of T-cells family member 2, Membrane-associated adapter molecule, NTAL, Non-T-cell activation linker, WBS15, WBSCR5, Williams-Beuren syndrome chromosomal region 15 protein
    Gene ID
    7462
    UniProt
    Q9GZY6
    Pathways
    Fc-epsilon Receptor Signaling Pathway, BCR Signaling
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