O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT) antibody

Antigen: O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT)

Synonyms: HRNT1, FLJ23071, MGC22921, O-GLCNAC, MGC80426, MGC69550

Clonality: Monoclonal (RL2)

Host: Mouse

Application: Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN120926

Additional Information

Alternative name: O-GlcNAc

Swiss-Prot: O15294

Immunogen: Pore complex-lamina fraction purified from rat liver nuclear envelopes

Isotype: IgG1  (Matching secondary antibodies)

Clone: RL2

Description: Diffusion of metabolites and small non-nuclear molecules as well as active, mediated import of protein and export of protein and RNA through the nuclear envelope occurs through nuclear pore complexes or NPC's. NPC's contain up to 100 different polypeptides which have a combined mass of about 125 megadaltons. The channel available for passive transport through the NPC is about 9-10 nm in diameter while carrier mediated changes in the NPC result in a ~25 nm channel used for larger, actively transported molecules. Of the 100 polypeptides, at least 8 of these are O-linked N-acetylglycosamine-modified in mammalian cells. All of the mammalian O-linked glycoproteins contain multiple copies of phenylalanine, glycine dipeptide repeats dispersed throughout part of their sequence. Studies indicate that the NPC O-linked glycoproteins have a direct role in nuclear protein import. Alternate Names: OGT , O-linked N-acetylglucosamine transferase 110 kDa subunit, UDP-N-acetylglucosamine- peptide N-acetylglucosaminyltransferase 110 kDa subunit.

Specificity: This antibody detects nuclear pore complex (NPC), cytoplasmic and intranuclear O-linked glycoproteins form human, mouse, and rat tissues.

Application Details

Application Notes: Immunofluorescence: 1/100, primarily labels NPC O-linked glycoproteins and has been successfully used on a wide variety of mammalian cells. Labeling occurs at the NPC, with most of the labeling at the cytoplasmic and/or nucleoplasmic margins, as well as within the nucleus. Immunohistochemistry on paraffin embedded sections: 1/200. Immunoprecipitation. Western Blot: 1/1000, recognizes up to eight different proteins from the NPC of approximately 210, 180, 145, 100, 63, 58, 54 and 45 kDa as well as other O-linked glycoproteins outside of the NPC. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 2.0 mg/ml

Buffer: PBS buffer with 0.05% Sodium Azide as preservative.

Storage: Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Restrictions: For Research Use only