G Protein-Coupled Receptor Kinase 1 (GRK1) antibody

Antigen: G Protein-Coupled Receptor Kinase 1 (GRK1)

Synonyms: RK, RHOK, GPRK1, Rk, Rhok, Gprk1, GRK1, CT4320, Gprk-1, DmelCG40129, CG40129, grk1

Clonality: Monoclonal (D11)

Host: Mouse

Reactivity: Cow (Bovine), Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN120951

Additional Information

Alternative name: Rhodopsin kinase

UniProt: Q15835

Immunogen: Full length human GRK1

Cross-Reactivity: Cow (Bovine), Human

Isotype: IgG1

Clone: D11

Description: The sensation of sight is the result of a cascade of events starting with the interaction of photoactivated rhodopsin with a protein called transducin. Rhodopsin kinase is a G-protein- coupled Ser/Thr kinase, also known as GRK1, which is a key element in the regulation of this cascade. Following phosphorylation by GRK1, arrestin is recruited to phospho-rhodopsin quenching its phototransductive activity by preventing further interaction with transducin. By breaking the cycle of phototransduction, GRK1 plays an important role in the restoration of the system for subsequent visual events.

Application Details

Application Notes: Immunofluorescence: 1/1000, staining of GRK1a/b in bovine retina with this antibody results in intense staining primarily in cone outer segments, but also in rod outer segments. Weak staining is observed in somata and synaptic terminals of cones and inner segments of rods. Immunohistochemistry on frozen sections: 1/1000. Immunoprecipitation. Western Blot: 1/1000, detects an ~60 kDa protein representing GRK 1a/b. ABIN120951 detects both in vitro expressed splice variants but was not able to detect significant amounts of GRK1b from human retina. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Purified

Buffer: PBS with 0.05% sodium azide as preservative.

Storage: Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Restrictions: For Research Use only

Images

Figure 1. Immunolocalization of GRK1a/b in bovine retina using ABIN120951.

Publications

Sonnenberg, Daams, Van der Valk et al.: "Development of mouse mammary gland: identification of stages in differentiation of luminal and myoepithelial cells using monoclonal antibodies and polyvalent antiserum against keratin." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 34, Issue 8, pp. 1037-46, 1986 (PubMed).

Sonnenberg, Hogervorst, Osterop et al.: "Identification and characterization of a novel antigen complex on mouse mammary tumor cells using a monoclonal antibody against platelet glycoprotein Ic." in: The Journal of biological chemistry, Vol. 263, Issue 28, pp. 14030-8, 1988 (PubMed).

Hemler, Crouse, Sonnenberg: "Association of the VLA alpha 6 subunit with a novel protein. A possible alternative to the common VLA beta 1 subunit on certain cell lines." in: The Journal of biological chemistry, Vol. 264, Issue 11, pp. 6529-35, 1989 (PubMed).

Sonnenberg, Modderman, Hogervorst: "Laminin receptor on platelets is the integrin VLA-6." in: Nature, Vol. 336, Issue 6198, pp. 487-9, 1989 (PubMed).

Sonnenberg, Janssen, Hogervorst et al.: "A complex of platelet glycoproteins Ic and IIa identified by a rat monoclonal antibody." in: The Journal of biological chemistry, Vol. 262, Issue 21, pp. 10376-83, 1987 (PubMed).