You are viewing an incomplete version of our website. Please click to reload the website as full version.

G Protein-Coupled Receptor Kinase 1 (GRK1) antibody

Details for Product No. ABIN120951, Supplier: Login to see New
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Cow (Bovine), Human
(65), (25), (18), (2), (2), (1)
(54), (11), (1)
Clonality (Clone)
Monoclonal ()
(2), (2), (2), (2), (2), (2), (1)
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(59), (39), (37), (5), (3), (2), (1), (1), (1)
Pubmed 5 references available
Supplier Login to see New
Catalog number from supplier Login to see New
Quantity 0.1 mg
Shipping to United States ( )
Immunogen Full length human GRK1
Clone D11
Isotype IgG1
Specificity This antibody detects G-protein-associated rhodopsin kinase 1a/b (GRK1a/b) from human and bovine tissues. This antibody does not cross-react with other G protein-coupled kinases. This antibody recognizes an N-terminal epitope of human GRK1 which is conserved in both GRK1a and GRK1b.
Purification Purified
Alternative Name Rhodopsin Kinase (GRK1 Antibody Abstract)
Background The sensation of sight is the result of a cascade of events starting with the interaction of photoactivated rhodopsin with a protein called transducin. Rhodopsin kinase is a G-protein-coupled Ser/Thr kinase, also known as GRK1, which is a key element in the regulation of this cascade. Following phosphorylation by GRK1, arrestin is recruited to phospho-rhodopsin quenching its phototransductive activity by preventing further interaction with transducin. By breaking the cycle of phototransduction, GRK1 plays an important role in the restoration of the system for subsequent visual events.Synonyms: G protein-coupled receptor kinase 1, GRK1, RHOK
Gene ID 6011
UniProt Q15835
Application Notes Immunofluorescence: 1/1000, staining of GRK1a/b in bovine retina with this antibody results in intense staining primarily in cone outer segments, but also in rod outer segments. Weak staining is observed in somata and synaptic terminals of cones and inner segments of rods. Immunohistochemistry on frozen sections: 1/1000. Immunoprecipitation. Western Blot: 1/1000, detects an approx. 60 kDa protein representing GRK 1a/b. ABIN120951 detects both in vitro expressed splice variants but was not able to detect significant amounts of GRK1b from human retina.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS with 0.05 % sodium azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.
Expiry Date 12 months
Supplier Images
Immunofluorescence (IF) image for anti-G Protein-Coupled Receptor Kinase 1 (GRK1) antibody (ABIN120951) Figure 1. Immunolocalization of GRK1a/b in bovine retina using ABIN120951.
Background publications Sonnenberg, Janssen, Hogervorst et al.: "A complex of platelet glycoproteins Ic and IIa identified by a rat monoclonal antibody." in: The Journal of biological chemistry, Vol. 262, Issue 21, pp. 10376-83, 1987 (PubMed).

Sonnenberg, Modderman, Hogervorst: "Laminin receptor on platelets is the integrin VLA-6." in: Nature, Vol. 336, Issue 6198, pp. 487-9, 1989 (PubMed).

Hemler, Crouse, Sonnenberg: "Association of the VLA alpha 6 subunit with a novel protein. A possible alternative to the common VLA beta 1 subunit on certain cell lines." in: The Journal of biological chemistry, Vol. 264, Issue 11, pp. 6529-35, 1989 (PubMed).

Sonnenberg, Hogervorst, Osterop et al.: "Identification and characterization of a novel antigen complex on mouse mammary tumor cells using a monoclonal antibody against platelet glycoprotein Ic." in: The Journal of biological chemistry, Vol. 263, Issue 28, pp. 14030-8, 1988 (PubMed).

Sonnenberg, Daams, Van der Valk et al.: "Development of mouse mammary gland: identification of stages in differentiation of luminal and myoepithelial cells using monoclonal antibodies and polyvalent antiserum against keratin." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 34, Issue 8, pp. 1037-46, 1986 (PubMed).

Catalog No. ABIN120951

Order hotline:

  • +1 877 302 8632
  • +1 888 205 9894 (TF)