Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) antibody

Antigen: Prolyl 4-Hydroxylase, beta Polypeptide (P4HB)

Synonyms: DSI, GIT, PDI, PHDB, PDIA1, PO4DB, PO4HB, PROHB, ERBA2L, P4Hbeta, Thbp, ERp59, Pdia1, PDIR, LOC100328595, p55, P4HB, DKFZp469E2031

Clonality: Monoclonal (RL90)

Host: Mouse

Reactivity: Hamster, Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN120990

Additional Information

Alternative name: PDI

Swiss-Prot: P07237

Immunogen: Purified rat PDI protein

Isotype: IgG2a  (Matching secondary antibodies)

Clone: RL90

Description: The three dimensional structure of many extracellular proteins is stabilized by the formation of disulphide bonds. Studies suggest that a microsomal enzyme known as Protein Disulphide- Isomerase (PDI) is involved in disulphide-bond formation and isomerization, as well as the reduction of disulphide bonds in proteins. PDI, which catalyses disulphide interchange between thiols and protein dilsulphides, has also been referred to as thiol:protein-disulphide oxidoreductase and as glutathione:insulin transhydrogenase because of its role in reduction of disulphide bonds. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the carboxy-terminus of PDI and other soluble endoplasmic reticulum (ER) resident proteins including the 78 and 94 kDa glucose regulated proteins (GRP78 and GRP94 respectively). The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.

Specificity: Clone RL90 detects protein disulphide-isomerase (PDI) from human, rat and mouse tissues as well as hamster cells. Other species not tested.

Application Details

Application Notes: Immunofluorescence: 1/100. Immunohistochemistry on frozen and formalin-fixed paraffin-embedded tissue sections: 1/100, in rat intestine a pattern consistent with cytoplasmic staining is obtained). Immunoprecipitation (has been shown to inhibit the activity of PDI in vitro. Western blot: 1/1,000, detects a protein at 59 kDa representing PDI from rat liver extract or a slightly higher protein at 61 kDa representing PDI from human liver extract.Clone RL90 has also been found to inhibit disulfide bond reduction of the HIV protein, gp120, at the cell surface of CHO cells and human lymphoid cells. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Buffer: PBS containing 1 mg/ml BSA and 0.05% sodium azide as preservative

Storage: Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: Cancer, Organelles

Restrictions: For Research Use only