Endothelin (Human) antibody

Overview

Antigen	Endothelin
Immunogen	Synthetic Peptide: C(15) H L D I I W(21).
Reactivity	Human
Antibody Type	Monoclonal
Format	Ascites
Isotype	IgG2b »Matching secondary antibodies
Clone	3B8C9
Host	Mouse
Specificity	Endothelins (ET) show potent constrictor activity in vascular and non-vascular smooth muscle. This family of 21-amino acid peptides exists in at least three isoforms- ET-1, ET-2, and ET-3, and is produced in endothelial and epithelial cells. ET's can mediate biological effects in cells and tissues, and have been shown to bind to an ET receptor in the lung, kidney, heart, and liver. This antibody detects endothelin (ET)-1, ET-2 and ET-3. This antibody shows no cross-reactivity with the peptides ET(15-20) and ET(15-22). This antibody has been successfully used in ELISA procedures. By ELISA this antibody is useful in solid-phase or competitive solution based procedures particularly in the study of enzymatic processing of ET, and in the identification of putative enzyme inhibitors. ABIN120991 can detect approximately 0.5 pmol/ml ET-1 by direct ELISA. ABIN120991 immunizing peptide corresponds to amino acid residues 15-21 of endothelin. This sequence is conserved between numerous species.

Application	Enzyme Immunoassay (EIA)
Application Notes	ELISA: 1/10000. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Buffer	This antibody is supplied as liquid, PBS-diluted ascites containing 0.05% sodium azide as preservative.
Storage	Store the antibody at –20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Research Area	Hormones
Restrictions	Research only

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EMBL Harvester	Search human, mouse or rat genome for antigen Endothelin (Bioinformatic Harvester (EMBL))