Endothelin 1 (EDN1) antibody
| Antigen |
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| Synonyms |
ET1, HDLCQ7, EDN1, EDN, ET-1, Edn1, et1, et-1, preproET, Et1, fb14d01, wu:fb14d01, PPET1, PPET-1 |
| Clonality |
Monoclonal (3B8C9) |
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Host
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Reactivity
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Application
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Radioimmunoassay (RIA) (12), Enzyme Immunoassay (EIA) (10), Immunohistochemistry (IHC) (7), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (7), Immunohistochemistry (Frozen Sections) (IHC (fro)) (6), ELISA (5), Western Blotting (WB) (5), Immunoradiometric Assay (IRMA) (2), ELISA (Capture) (1), Immunofluorescence (IF) (1), Immunoprecipitation (IP) (1)
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| Catalog no. |
ABIN120991 |
| Quantity |
0.1 ml |
| Price |
420.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Alternative name
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Endothelin-1
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Swiss-Prot
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P05305
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Immunogen
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Synthetic Peptide: C(15) H L D I I W(21) corresponding to amino acid residues 15-21 of Endothelin.
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Format
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Ascites
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Isotype
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IgG2b (Matching secondary antibodies)
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Clone
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3B8C9
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Description
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Endothelins (ET) show potent constrictor activity in vascular and non-vascular smooth muscle. This family of 21-amino acid peptides exists in at least three isoforms- ET-1, ET-2, and ET-3, and is produced in endothelial and epithelial cells. ET's can mediate biological effects in cells and tissues, and have been shown to bind to an ET receptor in the lung, kidney, heart, and liver.
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Specificity
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This antibody detects endothelin (ET)-1, ET-2 and ET-3. This antibody shows no cross-reactivity with the peptides ET(15-20) and ET(15-22). This sequence is conserved between numerous species
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Application Notes
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ELISA (1/10,000): Useful in solid-phase or competitive solution based procedures particularly in the study of enzymatic processing of ET, and in the identification of putative enzyme inhibitors. Can detect approximately 0.5 pmol/ml ET-1 by direct ELISA. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Buffer
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PBS containing 0.05% sodium azide as preservative.
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Storage
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Aliquot and store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf Life: One year from despatch.
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Research Area
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Hormones
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Restrictions
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For Research Use only
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Alternatives for antigen "Endothelin 1 (EDN1)", type "Antibodies"
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Hosts
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Rabbit (14), Mouse (9), Sheep (1)
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Reactivities
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Human (18), Pig (Porcine) (3), Cow (Bovine) (2), Rat (Rattus) (1), Sheep (Ovine) (1)
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Applications
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Radioimmunoassay (RIA) (12), Enzyme Immunoassay (EIA) (10), Immunohistochemistry (IHC) (7), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (7), Immunohistochemistry (Frozen Sections) (IHC (fro)) (6), ELISA (5), Western Blotting (WB) (5), Immunoradiometric Assay (IRMA) (2), ELISA (Capture) (1), Immunofluorescence (IF) (1), Immunoprecipitation (IP) (1)
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Conjugates
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Biotin (2)
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Epitopes
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C-Term (2), AA 22-38 (1)
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Alternatives 
