Heat Shock Protein 60 (HSP60) antibody

Antigen: Heat Shock Protein 60 (HSP60)

Synonyms: HLD4, CPN60, GROEL, HSP60, HSP65, SPG13, HuCHA60, 60kDa, Hsp60, 12, BP5, Cpn60, Dmhsp60, G62, Hsp60A, Mmp-P1, l(1)10Ac, l(1)BP5, l(1)G8, l(1)HM21, l(1)L12, l(1)dp025, DmelCG12101, CG12101, HEAT SHOCK PROTEIN 60, HEAT SHOCK PROTEIN 60-3B, HSP60-3B, 23.m06276

Clonality: Monoclonal (A57-B9)

Host: Mouse

Application: Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN120994

Additional Information

Alternative name: HSP60

Swiss-Prot: Q3KMQ9

Immunogen: Recombinant Chlamydia trachomatis HSP60 protein

Format: Ascites

Isotype: IgG1  (Matching secondary antibodies)

Clone: A57-B9

Description: Heat shock protein 60 (HSP60) is homologous to HSP65 (a major antigenic mycobacterial protein) and E. coli GroEL. HSP60, also referred to as chaperonin-60, P1 and mitonin, has been classified as a member of a family of proteins termed chaperonins which act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly. HSP60 is an abundant protein which is constitutively expressed and is induced by environmental stress. HSP60 exists as a large oligomer composed of 14~60 kDa subunits arranged as two stacked rings. Alternate Names: HSPD1, HSP60, HSP-60, CPN60, 60 kDa heat shock protein, Heat shock protein 60, 60 kDa chaperonin, Chaperonin 60, Mitochondrial matrix protein P1, P60 lymphocyte protein, HuCHA60.

Specificity: ABIN120994 detects heat shock protein 60 kDa (HSP60) from bacteria, all three species of Chlamydia HSP60 with minimal cross-reactivity with Borrelia burgdorferi HSP60. It does not react with E. coli GroEL or HeLa HSP60. Epitope mapping studies suggest that this antibody binds to a linear sequence of Chlamydia HSP60 amino acids 517-522.

Synonyms: HLD4, CPN60, GROEL, HSP60, HSP65, SPG13, HuCHA60, 60kDa, Hsp60, 12, BP5, Cpn60, Dmhsp60, G62, Hsp60A, Mmp-P1, l(1)10Ac, l(1)BP5, l(1)G8, l(1)HM21, l(1)L12, l(1)dp025, DmelCG12101, CG12101, HEAT SHOCK PROTEIN 60, HEAT SHOCK PROTEIN 60-3B, HSP60-3B, 23.m06276

Application Details

Application Notes: Immunocytochemistry: 1/500. Immunocytochemical staining of HSP60 in HeLa cells infected with Chlamydia with ABIN120994 results in staining of the elementary and reticulate bodies within the chlamydial inclusions. Immunoprecipitation. Western Blot: 1/1000, this antibody detects a single ~60 kDa band representing HSP60 from C. trachomatis lysate. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Buffer: This antibody is supplied as liquid, PBS-diluted ascites containing 0.05% sodium azide as preservative.

Storage: Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf Life: One year from despatch.

Research Area: Heat Shock Proteins

Restrictions: For Research Use only