Adaptor-Related Protein Complex 2, alpha 1 Subunit (AP2A1) antibody

Antigen: Adaptor-Related Protein Complex 2, alpha 1 Subunit (AP2A1)

Synonyms: ADTAA, CLAPA1, AP2-ALPHA, hip9, hypj, adtab, ap2a1, clapa2, MGC69489, AP2A1, adtaa, clapa1, ap2-alpha

Clonality: Monoclonal (AC1-M11)

Host: Mouse

Reactivity: Amphibian, Cow (Bovine), Chicken, Human, Mouse (Murine), Pig (Porcine), Primate, Rat (Rattus)

Application: Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN121006

Additional Information

Alternative name: AP2A1 / ADTAA (all alpha forms)

Swiss-Prot: O95782

Immunogen: Purified bovine brain adaptor complexes

Format: Ascites

Isotype: IgG2a  (Matching secondary antibodies)

Clone: AC1-M11

Description: Clathrin-mediated endocytosis is the pathway by which many receptors for nutrients and hormones are internalized to be recycled or down-regulated. During formation of clathrin coated membranes, clathrin co-assembles with heterotetrameric molecules known as assembly polypeptides (APs) or adaptors which form a layer of protein between the clathrin lattice and the membrane. There are two characterized adaptors AP1 and AP2. AP1 is associated with clathrin coated vesicles at the trans-Golgi network and AP2 is associated with the endocytic clathrin coated vesicles at the plasma membrane and has been shown to specifically interact with Src homologous and collagen-like (Shc) and epidermal growth factor (EGF) receptor. AP2 is composed of four subunits, two separate 100 kDa gene products with similar domain structures (alpha and beta adaptin) and a 50 and 17 kDa subunit. There are two alpha-adaptin genes, alphaA and alphaC which have a tissue specific pattern of expression.

Specificity: This antibody detects alpha-adaptin from bovine, chicken, frog, human, monkey, mouse, pig and rat. This antibody detects the products of both alpha-adaptin genes, alphaA and alphaC, as well as an alternatively spliced isoform of alphaA found in neurons.

Application Details

Application Notes: Immunofluorescence: 1/100, yields distinct punctate dots on the plasma membrane in transfected COS cells. Immunoprecipitation. Western Blot: 1/100, recognizes an ~100 kDa doublet representing the two isoforms of alpha- adaptin from pig brain extract. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Buffer: PBS containing 0.05% sodium azide as preservative.

Storage: Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Restrictions: For Research Use only

Images

Figure 1 shows immunolocalization of alpha-adaptin in NRK cells using ABIN121006.