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Details for Product No. ABIN121207

V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody

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Antigen
Synonyms
AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA, pkb, xAct, akt-1, v-akt, v-akt1, Akt, AKT1, ATAKT1, F18A8.2, F18A8_2, K+ transporter 1, POTASSIUM TRANSPORTER, AKT/PKB, Akt/PKB, Akt1, CG4006, D-Akt,  ... show more
Epitope
»Alternatives pSer473
Reactivity
»Alternatives Frog, Chicken, Human, Mouse (Murine)
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB)
Pubmed 4 references available
Catalog no. ABIN121207
Quantity 0.1 mg
Price
489.50 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 8 Business Days
Immunogen Synthetic peptide containing phosphorylated serines at amino acid residues 473 of human Akt1
Clone 104A282
Isotype IgG1
Purification Protein G Chromatography
Alternative Name AKT1 / PKB
Background Akt, protein kinase B (PKB), is a serine/threonine kinase which is involved in many cellular signaling pathways and acts as a transducer of many functions initiated by growth factor receptors that activat phosphtidylinositol 3-kinase (PI 3-kinase). The major activity of Akt/PKB is to mediate cell survival. Akt/PKB is also believed to be a critical factor in the genesis of cancer as the tumor suppressor PTEN was found to antagonise PI-3 kinase and Akt/PKB kinase activity. Akt/PKB phosphorylation is critical for it activity. The major phosphorylation sites required for Akt activation has been identified as threoinine 308 and serine 473. Serine 473 is phosphorylated by MAPKAP kinase 2.
UniProt P31749
Research Area Signaling, Protein Modifications, Kinases/Phosphatases
Application Notes Western blot: 2ug/ml. Immunoprecipitation: 1ug/ml. Recommended Positive Control : PDGF treated NIH 3T3. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 ml/ml
Buffer PBS containing 0.2% gelatin, 0.05% sodium azide
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: 6 month from despatch.
Expiry Date 6 months
Alessi, Andjelkovic, Caudwell et al.: "Mechanism of activation of protein kinase B by insulin and IGF-1." in: The EMBO journal, Vol. 15, Issue 23, pp. 6541-51, 1997 (PubMed).

Franke, Kaplan, Cantley: "PI3K: downstream AKTion blocks apoptosis." in: Cell, Vol. 88, Issue 4, pp. 435-7, 1997 (PubMed).

Cantley, Neel: "New insights into tumor suppression: PTEN suppresses tumor formation by restraining the phosphoinositide 3-kinase/AKT pathway." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 8, pp. 4240-5, 1999 (PubMed).

Kandel, Hay: "The regulation and activities of the multifunctional serine/threonine kinase Akt/PKB." in: Experimental cell research, Vol. 253, Issue 1, pp. 210-29, 1999 (PubMed).

Alternatives for antigen "V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1)", type "Antibodies"
Hosts (655), (147), (9), (5), (4), (3)
Reactivities (793), (523), (502), (175), (154), (117), (95), (21), (19), (5), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applications (590), (335), (205), (174), (143), (130), (117), (70), (31), (17), (17), (5), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Conjugates (21), (15), (13), (13), (13), (13), (13), (13), (13), (13), (13), (9), (7), (7), (7), (7), (1), (1), (1), (1), (1), (1)
Epitopes (124), (77), (45), (44), (43), (35), (30), (28), (27), (18), (16), (16), (9), (9), (7), (6), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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