V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody

Details for Product No. ABIN121207
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Antigen
Synonyms
AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA, pkb, xAct, akt-1, v-akt, v-akt1, Akt, AKT1, ATAKT1, F18A8.2, F18A8_2, K+ transporter 1, POTASSIUM TRANSPORTER, AKT/PKB, Akt/PKB, Akt1, CG4006, D-Akt,  ... show more
AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA, pkb, xAct, akt-1, v-akt, v-akt1, Akt, AKT1, ATAKT1, F18A8.2, F18A8_2, K+ transporter 1, POTASSIUM TRANSPORTER, AKT/PKB, Akt/PKB, Akt1, CG4006, D-Akt, DAKT1, DAKT1/PKB, DAkt, DAkt1, DPKB, DRAC-PK, DRAC-PK66, DRAC-PK85, Dakt, Dakt1, Dmel\\CG4006, Dpkb, PKB/AKT, PKB/Akt, PKB/dAKT, RacPK, akt, akt1, dAKT, dAKT/dPKB, dAKT1, dAkt, dAkt/PKB, dAkt1, dPKB, dakt, dakt1, l(3)04226, l(3)89Bq, p-Akt, pAkt show less
Epitope
pSer473
(129), (88), (54), (50), (48), (33), (32), (29), (28), (17), (16), (16), (11), (9), (9), (8), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Frog, Chicken, Human, Mouse (Murine)
(838), (537), (514), (167), (153), (115), (94), (21), (18), (4), (3), (1), (1), (1), (1)
Host
Mouse
(699), (144), (7), (5), (4), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(20), (14), (13), (13), (13), (13), (13), (13), (13), (13), (13), (9), (7), (7), (7), (7), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(638), (361), (240), (161), (139), (130), (124), (66), (44), (17), (11), (5), (5), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
Catalog no. ABIN121207
Quantity 0.1 mg
Price
500.50 $   Plus shipping costs $45.00
Shipping to United States (Change)
Availability Discontinued
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  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Synthetic peptide containing phosphorylated serines at amino acid residues 473 of human Akt1
Clone 104A282
Isotype IgG1
Purification Protein G Chromatography
Alternative Name AKT1 / PKB
Background Akt, protein kinase B (PKB), is a serine/threonine kinase which is involved in many cellular signaling pathways and acts as a transducer of many functions initiated by growth factor receptors that activat phosphtidylinositol 3-kinase (PI 3-kinase). The major activity of Akt/PKB is to mediate cell survival. Akt/PKB is also believed to be a critical factor in the genesis of cancer as the tumor suppressor PTEN was found to antagonise PI-3 kinase and Akt/PKB kinase activity. Akt/PKB phosphorylation is critical for it activity. The major phosphorylation sites required for Akt activation has been identified as threoinine 308 and serine 473. Serine 473 is phosphorylated by MAPKAP kinase 2.
UniProt P31749
Research Area Signaling, Protein Modifications, Kinases/Phosphatases
Application Notes Western blot: 2ug/ml. Immunoprecipitation: 1ug/ml. Recommended Positive Control : PDGF treated NIH 3T3. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 ml/ml
Buffer PBS containing 0.2% gelatin, 0.05% sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: 6 month from despatch.
Expiry Date 6 months
Supplier Images
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody Western blot analysis for Akt1 using ABIN121207at 2 ug/ml dilution against 10 ug/lane of untreated (lane 1) and PDGF treated (lane 2) NIH-3T3 lysate
General Alessi, Andjelkovic, Caudwell et al.: "Mechanism of activation of protein kinase B by insulin and IGF-1." in: The EMBO journal, Vol. 15, Issue 23, pp. 6541-51, 1997 (PubMed).

Franke, Kaplan, Cantley: "PI3K: downstream AKTion blocks apoptosis." in: Cell, Vol. 88, Issue 4, pp. 435-7, 1997 (PubMed).

Cantley, Neel: "New insights into tumor suppression: PTEN suppresses tumor formation by restraining the phosphoinositide 3-kinase/AKT pathway." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 8, pp. 4240-5, 1999 (PubMed).

Kandel, Hay: "The regulation and activities of the multifunctional serine/threonine kinase Akt/PKB." in: Experimental cell research, Vol. 253, Issue 1, pp. 210-29, 1999 (PubMed).

Hosts (699), (144), (7), (5), (4), (3)
Reactivities (838), (537), (514), (167), (153), (115), (94), (21), (18), (4), (3), (1), (1), (1), (1)
Applications (638), (361), (240), (161), (139), (130), (124), (66), (44), (17), (11), (5), (5), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Conjugates (20), (14), (13), (13), (13), (13), (13), (13), (13), (13), (13), (9), (7), (7), (7), (7), (1), (1), (1), (1), (1), (1)
Epitopes (129), (88), (54), (50), (48), (33), (32), (29), (28), (17), (16), (16), (11), (9), (9), (8), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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