V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3) antibody

Antigen: V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3)

Clonality: Monoclonal (66C1247)

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB)

Catalog no.: ABIN121209

Additional Information

Alternative name: AKT3 / PKB gamma

Swiss-Prot: Q9Y243

Immunogen: Synthetic peptide corresponding to amino acid residues 119-136 of human AKT3. AA Sequence:CSPTSQIDNIGEEEMDAS

Isotype: IgG1  (Matching secondary antibodies)

Clone: 66C1247

Description: AKT, protein kinase B (PKB), is a serine/threonine kinase which is involved in many cellular signaling pathways and acts as a transducer of many functions initiated by growth factor receptors that activat phosphtidylinositol 3-kinase (PI 3-kinase). The major activity of AKT/PKB is to mediate cell survival. AKT/PKB is also believed to be a critical factor in the genesis of cancer as the tumor suppressor PTEN was found to antagonise PI-3 kinase and AKT/PKB kinase activity. There are 3 known isoforms of this enzyme in mammalian cells (1/alpha, 2/beta and 3/gamma). AKT1, 2 and 3 contain a key regulatory serine phosphorylation site in the carboxy- terminal region of the protein. AKT/PKB phosphorylation is critical for it activity.

Specificity: This antibody is specific for AKT3. This sequence is identical in human, mouse, rat, cow, dog, and chicken. GenBank no. gi|4574744|gb|AAD24196.1|AF135794_1

Application Details

Application Notes: Western blotting (1-2 ug/ml). Recommended Positive Control: human kidney. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Buffer: PBS containing 0.05 % sodium azide, 0.05 % BSA

Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: Signaling

Restrictions: For Research Use only

Images

Western blot analysis for AKT3 using ABIN121209 at 2 ug/ml dilution against 30 ug/lane of human kidney lysate

Publications

Publications: Alessi, Andjelkovic, Caudwell et al.: "Mechanism of activation of protein kinase B by insulin and IGF-1." in: The EMBO journal, Vol. 15, Issue 23, pp. 6541-51, 1997 (PubMed).

Franke, Kaplan, Cantley: "PI3K: downstream AKTion blocks apoptosis." in: Cell, Vol. 88, Issue 4, pp. 435-7, 1997 (PubMed).

Cantley, Neel: "New insights into tumor suppression: PTEN suppresses tumor formation by restraining the phosphoinositide 3-kinase/AKT pathway." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 8, pp. 4240-5, 1999 (PubMed).

Kandel, Hay: "The regulation and activities of the multifunctional serine/threonine kinase Akt/PKB." in: Experimental cell research, Vol. 253, Issue 1, pp. 210-29, 1999 (PubMed).