Bacillus Anthracis Lethal Factor (LF) antibody

Antigen: Bacillus Anthracis Lethal Factor (LF)

Clonality: Monoclonal (5F502-2)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN121211

Additional Information

Alternative name: Bacillus anthracis lethal factor

UniProt: P15917

Immunogen: Synthetic peptide corresponding to amino acids 779-792 of the Anthrax LF protein (~90 kD)

Cross-Reactivity: Human

Isotype: IgG1

Clone: 5F502-2

Description: Anthrax PA (Protective Antigen) and Anthrax LF (Lethal Factor), along with Anthrax EF (Edema Factor), are toxins produced by Bacillus anthracis. All three of the proteins must be present for toxicity to occur. It begins with the binding of PA to its cell-surface receptor, ATR (Anthrax Toxin Receptor), at which point PA is cleaved and the resulting 63 kD carboxyl-terminal fragment binds to LF and EF. LF is a protease that is known to cleave members of the mitogen-activated protein kinase kinase (MAPKK) family2.

Specificity: This antibody is specific for Anthrax LF (Lethal Factor) protein.

Application Details

Application Notes: Western blot analysis (0.1 ug/ml, positive control: recombinant protein). In Daudi, a ~70 kDa band is observed. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.2 % gelatin and 0.05 % sodium azide

Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: Bacteria, Gram positive bacteria, Virology, Toxicology

Restrictions: For Research Use only

Images

Western blot analysis of Anthrax LF in recombinant protein using ABIN121211 at 0.1 ug/ml.

Publications

Collier, Young: "Anthrax toxin." in: Annual review of cell and developmental biology, Vol. 19, pp. 45-70, 2003 (PubMed).