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Activating Transcription Factor 2 (ATF2) (pThr71,pThr53) antibody

Antigen

Activating Transcription Factor 2 (ATF2)
Synonyms HB16, CREB2, TREB7, CRE-BP1, MGC111558, ATF2, MGC142504, CHN1, CG12850, CG3749, dATF-2, DmelCG30420, CG30420
Binding Site
Alternatives

pThr71,pThr53
Clonality Monoclonal (103C411)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB)
2 references available
Catalog no. ABIN121212
Quantity 0.1 mg  (0.5 mg/ml)
Price 396.00 $   Plus shipping costs $45.00
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anti-Activating Transcription Factor 2 (ATF2) (pThr71,pThr53) antibody

Additional Information
Application Details Publications Images Alternatives back to top

Alternative name ATF2
UniProt P15336
Immunogen This antibody was generated by immunizing mice with a synthetic peptide containing phosphorylated threonine at position 71 of human ATF-2.
Cross-Reactivity Human, Mouse (Murine)
Isotype IgG1
Clone 103C411
Description ATF-2 is a member of the group of bZip transcription factors. Heterodimer formation between members of the bZip group is common and is believed to add diversity to the cis-acting elements at which binding of the dimers is directed. Specifically, ATF-2 may dimerize with c-Jun, as occurs in response to E1a, and in so doing shift the binding preference of c-Jun toward ATF/CRE sites (1-3). Deletion analysis has indicated that the N-terminal region of ATF-2 containing threonine at residues 69 and 71 are essential for this purpose. These threonine residues are phosphorylated by JNK/SAPK for transcriptional activation.
Specificity This antibody reacts to Phosphorylated ATF2 (Thr71).

Application Details
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Application Notes Western blot: 2 ug/ml. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Concentration 0.5 mg/ml
Purification Purified
Buffer PBS containing 0.2% gelatin, 0.05% sodium azide
Storage Store the antibody undiluted at 2-8°C. DO NOT FREEZE! Shelf Life: 6 month from despatch.
Research Area Cell Cycle, Signaling, Kinases/Phosphatases
Restrictions For Research Use only

Publications
Application Details Images Alternatives back to top

Hai, Curran: "Cross-family dimerization of transcription factors Fos/Jun and ATF/CREB alters DNA binding specificity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 9, pp. 3720-4, 1991 (PubMed).

Benbrook, Jones: "Heterodimer formation between CREB and JUN proteins." in: Oncogene, Vol. 5, Issue 3, pp. 295-302, 1990 (PubMed).

Alternatives
Application Details Publications Images back to top

Alternatives for antigen "Activating Transcription Factor 2 (ATF2)", type "Antibodies"
Hosts Rabbit (261), Mouse (11), Goat (7), Sheep (7)
Reactivities Human (276), Rat (Rattus) (158), Mouse (Murine) (147), Cow (Bovine) (50), Horse (Equine) (43), Dog (Canine) (29), Chicken (22), Pig (Porcine) (20), Rabbit (18), Guinea Pig (5), Bird (Avian) (2), Cat (Feline) (1), Xenopus laevis (1)
Applications Western Blotting (WB) (157), Immunohistochemistry (IHC) (112), Immunofluorescence (IF) (94), ELISA (85), Immunoprecipitation (IP) (57), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (52), Dot Blot (Dot) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Immunocytochemistry (ICC) (4), Electrophoretic Mobility-Shift Assay (EMSA) (3), Immunoelectron Microscopy (IEM) (2), Flow Cytometry (FACS) (1), Functional Studies (Func) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
Conjugates Alexa Fluor 350 (9), Alexa Fluor 488 (9), Alexa Fluor 555 (9), Alexa Fluor 647 (9), PE,Cy5.5 (8), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1), Un-conjugated (1)
Epitopes Thr53 (12), pSer472 (9), pSer322 (8), pSer44 (8), pSer94 (8), C-Term (7), N-Term (7), pThr51 (7), pThr53 (6), pThr69, pThr71 (5), pThr71 (5), pSer498,pSer490 (4), pThr69, pThr51 (4), pSer112, pSer94 (3), pSer44, pSer62 (3), pSer490, pSer498 (3), pSer62, pSer44 (3), pSer62,pSer44 (3), pThr51, pThr69 (3), pThr53, pThr71 (3), pThr55, pThr73 (3), pThr69,pThr51 (3), pThr71, pThr53 (3), pThr73, pThr55 (3), Ab-112 or 94 (2), Ab-62 or 44 (2), Ab-69 or 51 (2), Ab-71 or 53 (2), Ab-73 or 55 (2), Internal Region (2), Ser44 (2), Ser94 (2), pSer112 or 94 (2), pSer112,pSer94 (2), pSer62 or 44 (2), pSer94, pSer112 (2), pThr69 or 51 (2), pThr71 or 53 (2), pThr71,pThr53 (2), pThr73 or 55 (2), pThr73,pThr55 (2), AA 13-27 (1), AA 95-211 (1), Phosphospecific (1), Ser112, Ser94 (1), Ser62 (1), Ser62, Ser44 (1), Ser69, Ser71 (1), Ser94, Ser112 (1), Thr51 (1), Thr71, Thr51 (1), Thr73, Thr55 (1), Thr99, Thr51 (1), pSer112 (1), pSer44,pSer62 (1), pSer480 (1), pSer490,pSer498 (1), pSer62 (1), pSer730 (1), pSer90 (1), pSer94, pSer119 (1), pSer94,pSer112 (1), pThr286 (1), pThr34 (1), pThr51,pThr69 (1), pThr53,pThr71 (1), pThr55,pThr73 (1), pThr69 (1), pThr69,pThr51,pThr71,pThr53 (1), pThr73 (1), pTyr412 (1)