You are viewing an incomplete version of our website. Please click to reload the website as full version.

SH3-Domain GRB2-Like Endophilin B1 (SH3GLB1) antibody

Details for Product No. ABIN121215, Supplier: Login to see New
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

(101), (34), (29), (2), (1), (1), (1), (1), (1)
(78), (16), (8), (1)
Clonality (Clone)
Monoclonal ()
(4), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Immunoprecipitation (IP), Western Blotting (WB)
(75), (42), (38), (23), (11), (9), (6), (5), (3), (2)
Pubmed 2 references available
Supplier Login to see New
Catalog number from supplier Login to see New
Quantity 0.1 mg
Shipping to United States ( )
Immunogen Full-length human Bif-1 recombinant protein
Clone 30A882-1-1
Isotype IgG1
Purification Protein G chromatography
Alternative Name Endophilin-B1 (SH3GLB1 Antibody Abstract)
Background The Bcl-2 family consists of anti-apoptotic (or cell survival) genes, such as Bcl-2, Bcl-XL and pro- apoptotic (or cell death) genes including Bax and BAD. In addition to its anti-apoptotic effect, Bcl-2 also inhibits progression through the cell cycle. Functional interactions between family members as well as binding to other cellular proteins modulate their activities. Bcl-2 family members are important in normal tissue development, homeostasis, and disease states. Bcl-2 prevents cell death by forming heterodimers with Bax and Bad (1). Bax promotes cell death by forming homodimers, which can be blocked by forming heterodimers with Bcl-2 or BCL-XL. Recently, using yeast two-hybrid system Cuddeback et al (product citation 2) have identified a cDNA encoding a 365 amino acid protein, which interacts physically with Bax protein and influences cell life and death. Bif-1 contains a Src homology 3 (SH3) domain in the C-terminus. It does not contain any of the conserved Bcl-2 homology (BH) domains of the Bcl-2 family proteins. Human Bif-1 protein shares 96% and 42% identity at the amino acids level with mouse and C. elegans, respectively. Overexpression of Bif-1 in transfected cell lines promotes Bax conformational change, caspase activation, and apoptotic cell death following growth factor withdrawal. Bif-1 RNA is abundantly expressed in heart, skeletal muscle, kidney, and placenta.
Application Notes Western blot: 1-2 ug/ml. IP: See Takahashi et al, 2007 for details. Jurkat cell lysate can be used as positive control. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS containing 0.05 % BSA and 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-SH3-Domain GRB2-Like Endophilin B1 (SH3GLB1) antibody (ABIN121215) Western blot analysis for Bif-1 using ABIN121215 at 2 ug/ml dilution against 10 ug of...
General Cuddeback, Yamaguchi, Komatsu et al.: "Molecular cloning and characterization of Bif-1. A novel Src homology 3 domain-containing protein that associates with Bax." in: The Journal of biological chemistry, Vol. 276, Issue 23, pp. 20559-65, 2001 (PubMed).

Reed: "Bcl-2 and the regulation of programmed cell death." in: The Journal of cell biology, Vol. 124, Issue 1-2, pp. 1-6, 1994 (PubMed).

Catalog No. ABIN121215

Order hotline:

  • +1 877 302 8632
  • +1 888 205 9894 (TF)