Caspase 14, Apoptosis-Related Cysteine Peptidase (CASP14) antibody

Antigen: Caspase 14, Apoptosis-Related Cysteine Peptidase (CASP14)

Synonyms: MICE, mini-ICE, MGC123948, MGC123949, MGC119078, MGC119079, CASP14, LOC100101622

Clonality: Monoclonal (70A1426)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB)

Catalog no.: ABIN121216

Additional Information

Alternative name: Caspase-14

UniProt: O89094

Immunogen: Synthetic peptide corresponding to amino acids 2-18 of mouse caspase-14

Cross-Reactivity: Human, Mouse (Murine)

Isotype: IgG

Clone: 70A1426

Description: Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Caspase-14 is a newly identified member of Caspase family. It has conserved active site pentapeptide, QACRG. However, no proteolytic or biological activities have been identified so far. It's high expression in embryonic tissues and limited expression in adult tissues suggests that it may have some role during ontogenesis.

Specificity: The antibody recognizes Caspase-14.

Application Details

Application Notes: Western blotting (2-4 ug/ml): in mouse spleen, a 30 kDa band is observed. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.02% sodium azide

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: Apoptosis/Necrosis, Autophagy

Restrictions: For Research Use only

Images

Western blot analysis for Caspase-14 using ABIN121216 at 2 ug/ml dilution against 10 ug of mouse spleen cell lysate

Publications

Cohen: "Caspases: the executioners of apoptosis." in: The Biochemical journal, Vol. 326 ( Pt 1), Issue 5693, pp. 1-16, 1997 (PubMed).

Hu, Snipas, Vincenz et al.: "Caspase-14 is a novel developmentally regulated protease." in: The Journal of biological chemistry, Vol. 273, Issue 45, pp. 29648-53, 1998 (PubMed).

Ahmad, Srinivasula, Hegde et al.: "Identification and characterization of murine caspase-14, a new member of the caspase family." in: Cancer research, Vol. 58, Issue 22, pp. 5201-5, 1998 (PubMed).