Search, Find and Buy Antibodies, ELISA Kits and Proteins.
Loading Loading
Order hotline:
phone +1 404 474 4654
fax +1 888 205 9894 (TF)
Details for Product No. ABIN121218

Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) antibody

Request
Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms
xcpp32, Lice, CPP32, SCA-1, CPP32B, CC3, CG14902, Cas3, Casp 3, Casp3, Caspase-3, DECAY, Decay, Dmel\\CG14902, Drosophila executioner caspase related to Apopain/Yama, caspase 3, caspase-3, CG7788, DRI ... show more
Reactivity
»Alternatives Human, Mouse (Murine), Rat (Rattus)
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Pubmed 3 references available
Catalog no. ABIN121218
Quantity 0.1 mg
Price
489.50 $   Plus shipping costs $45.00
Shipping to
Availability Discontinued
Immunogen Full-length human Caspase-3 protein
Clone 31A1067
Isotype IgG1
Purification Protein G Chromatography.
Alternative Name Caspase-3
Background Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis (1). The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Gene for Caspase-3 also known as Yama, CPP32, and apopain codes for a 32-kDa protein (2-4). Caspase-3 cleaves the death substrate poly(ADP-ribose) polymerase (PARP) to a specific 85 kDa form observed during apoptosis and is inhibitable by the CrmA protein. Other Caspase-3 substrates include DNA-PK, actin, GAS2, and procaspase-6, etc. (5). Caspase-3 is activated by cleavage events at Asp-28/Ser-29 (between N-terminal pro-domain) and Asp-175/Ser-176 (between large and small subunits) to generate a large subunit of 17-kDa and a small subunit of 12-kDa (3). Alternate Names: CASP3, Yama protein, Apopain, CPP32, CPP-32, SCA-1, FocusOn51 , Caspase 3, Cysteine protease CPP32, CASP 3, SREBP cleavage activity 1.
UniProt P42574
Research Area Apoptosis/Necrosis, Autophagy
Application Notes Immunohistochemistry on frozen (ref.6) and paraffin embedded (ref.7) sections. Recommended Positive Control: Staurosporine (2 mM) treated HeLa, Jurkat, Raw, NIH3T3. Western blot: 2-3 ug/ml. Application Notes ABIN121218 detects both pro caspase-3 (~32 kDa) and the large subunit of the active/cleaved form (~14-21 kDa) of Caspase-3. The large subunit of the cleaved form may appear as one or two or even as a stack of bands depending on the presence or absence of the Caspase-3 pro- domain. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. 1 / 3
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS containing 0.05% BSA and 0.05% Sodium Azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Expiry Date 12 months
Tewari, Quan, ORourke et al.: "Yama/CPP32 beta, a mammalian homolog of CED-3, is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymerase." in: Cell, Vol. 81, Issue 5, pp. 801-9, 1995 (PubMed).

Shi, Chen, MacDonald et al.: "Activation of an interleukin 1 converting enzyme-dependent apoptosis pathway by granzyme B." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 20, pp. 11002-7, 1996 (PubMed).

Cohen: "Caspases: the executioners of apoptosis." in: The Biochemical journal, Vol. 326 ( Pt 1), Issue 5693, pp. 1-16, 1997 (PubMed).

Alternatives for antigen "Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3)", type "Antibodies"
Hosts (233), (57), (7), (2)
Reactivities (275), (93), (83), (25), (23), (22), (9), (9), (8), (7), (6), (6), (5), (4), (3), (1), (1), (1), (1), (1)
Applications (241), (99), (80), (78), (63), (17), (17), (15), (11), (11), (10), (6), (4), (3), (2), (2), (2), (1)
Conjugates (11), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (31), (19), (13), (13), (8), (8), (7), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Beacon