Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) antibody

Antigen: Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3)

Synonyms: CC3, Lice, Yama, CPP32, Apopain, Caspase-3, A830040C14Rik, Cas3, Casp3, DECAY, Drosophila executioner caspase related to Apopain/Yama, caspase 3, DmelCG14902, CG14902, DRICE, ICE, DmelCG7788, CG7788, casp3, xcpp32, CASP3

Clonality: Monoclonal (31A893)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN121219

Additional Information

Alternative name: Caspase-3

UniProt: P42574

Immunogen: 6xHis tag full-length human Caspase-3 protein

Cross-Reactivity: Human

Isotype: IgG1

Clone: 31A893

Description: Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis (1). The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Gene for Caspase-3 also known as Yama, CPP32, and apopain codes for a 32-kDa protein (2-4). Caspase-3 cleaves the death substrate poly(ADP-ribose) polymerase (PARP) to a specific 85 kDa form observed during apoptosis and is inhibitable by the CrmA protein. Other Caspase-3 substrates include DNA-PK, actin, GAS2, and procaspase-6, etc. (5). Caspase-3 is activated by cleavage events at Asp-28/Ser-29 (between N-terminal pro-domain) and Asp-175/Ser-176 (between large and small subunits) to generate a large subunit of 17-kDa and a small subunit of 12-kDa (3).

Synonyms: CC3, Lice, Yama, CPP32, Apopain, Caspase-3, A830040C14Rik, Cas3, Casp3, DECAY, Drosophila executioner caspase related to Apopain/Yama, caspase 3, DmelCG14902, CG14902, DRICE, ICE, DmelCG7788, CG7788, casp3, xcpp32, CASP3

Application Details

Application Notes: Western blot analysis (2.0 ug/ml). Positive control: HL60 or staurosporine treated apoptotic HeLa. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.2% gelatin and 0.05% sodium azide

Storage: Store the antibody at 2-8°C for up to one month or at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: Apoptosis/Necrosis, Autophagy

Restrictions: For Research Use only

Images

Western blot analysis for human Caspase-3 using HL60 lysates with ABIN121219 at 2 ug/ml dilution. ABIN121219 only detects a 32 kD caspase-3 corresponding to pro-caspase-3.

Publications

Tewari, Quan, ORourke et al.: "Yama/CPP32 beta, a mammalian homolog of CED-3, is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymerase." in: Cell, Vol. 81, Issue 5, pp. 801-9, 1995 (PubMed).

Shi, Chen, MacDonald et al.: "Activation of an interleukin 1 converting enzyme-dependent apoptosis pathway by granzyme B." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 20, pp. 11002-7, 1996 (PubMed).