Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) antibody

Details for Product No. ABIN121219
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Antigen
Reactivity
Human
(309), (118), (94), (19), (18), (18), (6), (5), (4), (4), (4), (3), (2), (2), (2), (1)
Host
Mouse
(276), (60), (7), (2), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(10), (8), (6), (4), (4), (4), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(264), (118), (100), (91), (54), (31), (27), (19), (19), (14), (10), (4), (2), (2), (2)
Pubmed 2 references available
Quantity 0.1 mg
Shipping to United States (Change)
Availability Discontinued
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Catalog No. ABIN121219
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Immunogen 6xHis tag full-length human Caspase-3 protein
Clone 31A893
Isotype IgG1
Purification Protein G affinity chromatography
Alternative Name Caspase-3
Background Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis (1). The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Gene for Caspase-3 also known as Yama, CPP32, and apopain codes for a 32-kDa protein (2-4). Caspase-3 cleaves the death substrate poly(ADP-ribose) polymerase (PARP) to a specific 85 kDa form observed during apoptosis and is inhibitable by the CrmA protein. Other Caspase-3 substrates include DNA-PK, actin, GAS2, and procaspase-6, etc. (5). Caspase-3 is activated by cleavage events at Asp-28/Ser-29 (between N-terminal pro-domain) and Asp-175/Ser-176 (between large and small subunits) to generate a large subunit of 17-kDa and a small subunit of 12-kDa (3).
UniProt P42574
Research Area Apoptosis/Necrosis, Autophagy
Application Notes Western blot analysis (2.0 ug/ml). Positive control: HL60 or staurosporine treated apoptotic HeLa. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS containing 0.2% gelatin and 0.05% sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2-8°C for up to one month or at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Expiry Date 12 months
Supplier Images
anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) antibody Western blot analysis for human Caspase-3 using HL60 lysates with ABIN121219 at 2 ug/ml dilution. ABIN121219 only detects a 32 kD caspase-3 corresponding to pro-caspase-3.
General Tewari, Quan, ORourke et al.: "Yama/CPP32 beta, a mammalian homolog of CED-3, is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymerase." in: Cell, Vol. 81, Issue 5, pp. 801-9, 1995 (PubMed).

Shi, Chen, MacDonald et al.: "Activation of an interleukin 1 converting enzyme-dependent apoptosis pathway by granzyme B." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 20, pp. 11002-7, 1996 (PubMed).

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